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Mycobacterium tuberculosis normoxia hypoxia reactivation with ActivX-desthiobiotin FP probe

ABSTRACT: Lysates of Mycobacterium tuberculosis (H37Rv auxotroph mc(2)6020) grown under various conditions (normoxia, hypoxia, reactivation from hypoxia) probed the serine hydrolase probe with ActivX-desthiobiotin FP.

INSTRUMENT(S): Exactive Plus Orbitrap (Thermo Scientific)

ORGANISM(S): Mycobacterium Tuberculosis (ncbitaxon:1773)

SUBMITTER: Kimberly E. Beatty

PROVIDER: MSV000080238 | MassIVE | Mon Oct 10 21:04:00 BST 2016

SECONDARY ACCESSION(S): PXD005127

REPOSITORIES: MassIVE

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Publications

Small-Molecule Probes Reveal Esterases with Persistent Activity in Dormant and Reactivating Mycobacterium tuberculosis.

Tallman Katie R KR Levine Samantha R SR Beatty Kimberly E KE

ACS infectious diseases 20161013 12

Mycobacterium tuberculosis (Mtb) is the deadliest bacterial pathogen in the world. An estimated one-third of humans harbor Mtb in a dormant state. These asymptomatic, latent infections impede tuberculosis eradication due to the long-term potential for reactivation. Dormant Mtb has reduced enzymatic activity, but hydrolases that remain active facilitate pathogen survival. We targeted Mtb esterases, a diverse set of enzymes in the serine hydrolase family, and studied their activities using both ac ...[more]

PMID: 27690385

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