Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Velos
ORGANISM(S): Rattus Rattus (ncbitaxon:10117)
SUBMITTER: Jesper V. Olsen
PROVIDER: MSV000080552 | MassIVE | Thu Feb 16 18:09:00 GMT 2017
SECONDARY ACCESSION(S): PXD002431
REPOSITORIES: MassIVE
Secher Anna A Kelstrup Christian D CD Conde-Frieboes Kilian W KW Pyke Charles C Raun Kirsten K Wulff Birgitte S BS Olsen Jesper V JV
Nature communications 20160504
Large-scale mass spectrometry-based peptidomics for drug discovery is relatively unexplored because of challenges in peptide degradation and identification following tissue extraction. Here we present a streamlined analytical pipeline for large-scale peptidomics. We developed an optimized sample preparation protocol to achieve fast, reproducible and effective extraction of endogenous peptides from sub-dissected organs such as the brain, while diminishing unspecific protease activity. Each peptid ...[more]