Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Velos
ORGANISM(S): Rattus Rattus (black Rat)
TISSUE(S): Brain
SUBMITTER: Christian Kelstrup
LAB HEAD: Jesper V. Olsen
PROVIDER: PXD002431 | Pride | 2016-05-03
REPOSITORIES: Pride
Items per page: 1 - 5 of 33 |
Nature communications 20160504
Large-scale mass spectrometry-based peptidomics for drug discovery is relatively unexplored because of challenges in peptide degradation and identification following tissue extraction. Here we present a streamlined analytical pipeline for large-scale peptidomics. We developed an optimized sample preparation protocol to achieve fast, reproducible and effective extraction of endogenous peptides from sub-dissected organs such as the brain, while diminishing unspecific protease activity. Each peptid ...[more]