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Hydrolysis and oxidation of maleimide alkylated peptides

ABSTRACT: These data show that maleimide-alkylated peptides (MAPs) are oxidized and hydrolysed under sample preparation conditions common for proteomic studies. Gel electrophoresis, gel staining, and proteolytic digestion buffers were optimised to minimise these modifications. Purified glutathione alkylated with biotin maleimide (Sigma Aldrich), was the peptide used in these investigations.

INSTRUMENT(S): TripleTOF 5600, TOF/TOF 5800

ORGANISM(S): Bos Taurus (ncbitaxon:9913) Gallus Gallus (ncbitaxon:9031)

SUBMITTER: Amber Boyatzis

PROVIDER: MSV000080667 | MassIVE | Thu Mar 23 08:07:00 GMT 2017

REPOSITORIES: MassIVE

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