Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Porcine sinoatrial node extracellular matrix

ABSTRACT: comparison of pooled decellularized porcine left ventricle (LV) or sinoatrial node (SAN) extracellular matrix digested with either trypsin or elastase

INSTRUMENT(S): Q Exactive

ORGANISM(S): Porcine

SUBMITTER: Deborah K. Lieu

PROVIDER: MSV000081000 | MassIVE | Tue Apr 25 14:34:00 BST 2017

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Porcine brain tissue-derived decellularized extracellular matrix LC-MS/MS

Project description:The protein composition of porcine brain tissue-derived decellularized extracellular matrix was identified.

2023-11-24 | PXD046191 | Pride

Extracellular matrix hydrogel derived from decellularized tissues enables endoderm organoids culture

Project description:Organoids have huge therapeutic potential and are increasingly opening up new avenues within regenerative medicine. However, the clinical application is greatly limited by the lack of effective GMP-complaint systems for organoid expansion in culture. Here, we envisage that the use of extracellular matrix hydrogels derived from decellularized tissues (DT) can provide the instructive native environment necessary to direct cell growth. These gels possess the biochemical signature of tissue-specific ECM, and have the potential for clinical translation due to their natural, non-carcinogenic origin. We show that gels developed from decellularized porcine small intestinal mucosa have similar physiological range and mechanical properties to commercially available gels. ECM-derived gels enable formation and growth of endoderm-derived organoids and, moreover, supports in vivo organoid growth. The development of these ECM-derived hydrogels opens up the potential for human organoids to be used clinically.

2019-11-05 | GSE135108 | GEO

Extracellular matrix hydrogel derived from decellularized tissues enables endoderm organoids culture

Project description:Organoids have extensive therapeutic potential and are increasingly opening up new avenues within regenerative medicine. However, the clinical application is greatly limited by the lack of effective GMP-compliant systems for organoid expansion in culture. Here, we envisage that the use of extracellular matrix hydrogels derived from decellularized tissues (DT) can provide an environment capable of directing cell growth. These gels possess the biochemical signature of tissue-specific ECM, and have the potential for clinical translation. Gels from decellularized porcine small intestinal mucosa/submucosa enable formation and growth of endoderm-derived human organoids, such as gastric, liver, pancreas, and small intestine. ECM gels could be used for direct organoids derivation from human biopsies, multiple passages with transcriptomic signature comparable to gold standard culture system, and in vivo organoid delivery. The development of these ECM-derived hydrogels opens up the potential for human organoids to be used clinically.

2019-11-05 | GSE138611 | GEO

An extracellular matrix-driven positive feedback loop regulates translation in Idiopathic Pulmonary Fibrosis

Project description:Analysis of gene expression of lung fibroblasts seeded onto decellularized extracellular matrix (ECM). Experiment had 2x2 design where fibroblasts from idiopathic pulmonary fibrosis (IPF) or control patients were seeded onto decelluarized lung tissue from IPF or control patients allowing for determination of gene expression differences that were driven by IPF ECM and which differences were driven by the IPF fibroblast. Lung fibroblasts from 5 patients with idiopathic pulmonary fibrosis and 5 control patients were cultured on decellularized ECM from IPF or control lung. Total RNA and polyribosome RNA were isolated after the cells were cultured on the decellularized ECM for 18 hours. When possible, a control cell line and a diseased cell line were cultured (and processed) simultaneously to minimize the effect of experimental variance induced by running the experiment at different times.Samples with the same batch number (provied in the sample 'characteristics' field) were cultured and processed at the same time.

2014-03-04 | E-GEOD-45686 | biostudies-arrayexpress

Transcriptomic profiling of murine aneurysm formation after porcine pancreatic elastase infusion

Project description:In the present work, we aimed to investigate the changes in total RNA expression after porcine pancreatic elastase (PPE) instillation in C57BL/6 mice.

2022-01-01 | GSE153097 | GEO

Tissue extracellular matrix hydrogels as alternative to Matrigel for culturing gastrointestinal organoids

Project description:To determine the performance of the decellularized GI tissue ECM hydrogel, we analyzed differences in mRNA expression levels in native gastric tissues, gastric organoids cultured in Matrigel and those cultured in decellularized gastric ECM hydrogel. We also compared mRNA expression levels of native small intestinal tissues, small intestinal organoids grown in Matrigel and those grown in decellularized intestinal ECM hydrogel.

2022-01-30 | GSE165309 | GEO

Phosphoproteomics evaluation of the effect of GLP-1 on the porcine pacemaker

Project description:GLP-1 induces an increase in heart rate, but its mechanism remains elusive. We used a phosphoproteomic approach to evaluate the effect of GLP-1 on the porcine sinus node. Tissue samples from the sinus node region and left ventricle were collected from eight animals following a 60-minute infusion with either GLP-1 or saline. We employed a tandem mass tag (TMT) labeling strategy to multiplex samples, enriched for phosphorylated peptides by titanium dioxide chromatography, and pre-fractionated the resulting peptide pool prior to LC-MS/MS measurements on an Orbitrap Ascend Tribrid mass spectrometer.

2024-06-10 | PXD042583 | Pride

Proteomic analysi of decellularized porcine pancreas

Project description:The extracellular matrix is an important part of the cellular microenvironment regulating various cellular functions. Decellularized matrices present a valuable strategy for recreating the cellular niche in vitro and implementing matrix signaling in cell culture models. However, the tissue-specificity of decellularized matrices is little considered in most in vitro models. Here, we present the generation of a porcine-derived pancreas-specific decellularized extracellular matrix scaffold (d-PanMa) and show the impact of scaffold-specific cues on stem cell culture. The proteomic analysis in this work compare the proteome of porcine pancreas (PanMa), intestine (SISser) and lung (lungMa) both in its native and decellularized form. The analysis verify the enrichment of important matrisome proteins in the decellular matrices and provide a detailed map of the protein composition and abundance across the matrices.

2020-03-23 | PXD014356 | Pride

Delivery of a tissue derived extracellular matrix gel improves recovery following both acute and chronic disuse muscle injuries.

Project description:To evaluate decellularized skeletal muscle extracellular matrix hydrogel effectiveness in treating disuse atrophy using a mouse hindlimb suspension (HU) model. Male C57BL/6J mice were subjected to 5 days of hind limb unloading then received intramuscular matrix gel on the left TA. We then performed gene expression profiling analysis using data obtained from RNA-seq from mouse TA muscles (n=4 for matrix gel treatment, n=4 for PBS control , and n=4 for uninjured control) at day 7 post matrix gel injection

2023-01-02 | GSE220641 | GEO

Porcine LC-MSMS - Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue Culture

Project description:Porcine breast fatty extracellular matrix proetiens has been extracted after decellularization of fatty tissue with conventional method (PFT-1) using triton X-100 and sodium dodecyl sulfate and extracted with modified methods keeping natural protein in their natural condition using X-100, urea, thiourea etc

2019-11-12 | PXD011011 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data