Proteomics

Dataset Information

0

Analysis of peripheral neuron protein expression in the absence of Ikbkap/Elp1

ABSTRACT: Familial dysautonomia (FD) results from mutation in IKBKAP/ELP1, a gene encoding the scaffolding protein for the Elongator complex. This highly conserved complex is required for the methoxy-carbonyl-methyl (mcm5) modification of uridines located in the wobble position of tRNA molecules (U34). In FD, the peripheral nervous system is particularly devastated by loss of IKBKAP. Here we investigate protein expression within the dorsal root ganglia (DRG) of a mouse model of FD. In this model, Ikbkap is selectively deleted in the neural crest lineage using a Wnt1-Cre transgene and floxed Ikbkap. DRG were collected and pooled from seven Ikbkap conditional knockout (Wnt1-Cre;IkbkapLoxP/LoxP) and seven control E17.5 mice and analyzed via UHPLC-MS/MS.

OTHER RELATED OMICS DATASETS IN: PRJNA318069PRJNA318068GSE80165

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Lynn D George  

PROVIDER: MSV000081568 | MassIVE |

SECONDARY ACCESSION(S): PXD007869

REPOSITORIES: MassIVE

Json Xml

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1
altmetric image

Publications

Elongator and codon bias regulate protein levels in mammalian peripheral neurons.

Goffena Joy J   Lefcort Frances F   Zhang Yongqing Y   Lehrmann Elin E   Chaverra Marta M   Felig Jehremy J   Walters Joseph J   Buksch Richard R   Becker Kevin G KG   George Lynn L  

Nature communications 20180301 1

Familial dysautonomia (FD) results from mutation in IKBKAP/ELP1, a gene encoding the scaffolding protein for the Elongator complex. This highly conserved complex is required for the translation of codon-biased genes in lower organisms. Here we investigate whether Elongator serves a similar function in mammalian peripheral neurons, the population devastated in FD. Using codon-biased eGFP sensors, and multiplexing of codon usage with transcriptome and proteome analyses of over 6,000 genes, we iden  ...[more]

Similar Datasets

Transcriptomics Whole transcriptome analysis of FACS purified somatosensory neuron subtypes and whole dorsal root ganglia tissue
2014-12-23 | E-GEOD-55114 | biostudies-arrayexpress
Transcriptomics Expression data from FACS purified nociceptor neurons from peripheral sensory ganglia
2013-08-28 | E-GEOD-46546 | biostudies-arrayexpress
Transcriptomics Comparison of CGD transgene-expressing and non-expressing tumor cells from a MPNST allograft model
2021-01-31 | GSE152752 | GEO
Proteomics Quantification of oxidized proteins in wild type and Nox2 deficient mouse dorsal root ganglia
2020-10-13 | PXD018085 | Pride
Proteomics Quantitative characterization of the neuropeptide level changes in dorsal horn and dorsal root ganglia regions of the murine itch models
2020-01-23 | PXD015949 | Pride
Proteomics Proteomics on Nav1.8-positive nociceptors of mouse DRG
2022-10-03 | PXD034447 | Pride
Proteomics Dorsal root ganglia(DRG) proteomic analysis
2023-05-09 | PXD041382 | Pride
Proteomics Proteomic analysis in the gigaxonin null mouse model
2023-04-24 | MSV000091788 | MassIVE
Transcriptomics RNA-Seq data for dorsal root ganglia of RECTAS-treated ELP1/IKBKAP (IVS20+6T>C) transgenic mice
2021-03-10 | GSE161109 | GEO
Transcriptomics Intersectionally labeled Drd2-Pet1 single-neuron RNA-seq
2016-10-08 | GSE87758 | GEO