Dataset Information

Genus wide venom proteomics of cobras (Naja)

ABSTRACT: Genus-wide proteomics analysis of cobra (Naja) venoms. For top-down analysis, venom samples were reduced with TCEP and measured via HPLC-MS/MS (Q-Exactive) . Spectrum-Protein matching was performed with TopPic1.1 against a genus wide translated transcriptome database and NCBI protein entries from the najas. For bottom-up analysis, HPLC separated fraction of venom were dried down, reduced with triethylphosphine and alkylated with 2-iodoethanol. HPLC-MS/MS experiments were performed on normalflow UHPLC-QTOF system (AB Sciex 5600 TripleTOF). Data analysis (PSM) was performed ProteinPilot version 4.0 and the transcriptome derived protein sequence database.

INSTRUMENT(S): Q-Exactive, TripleTOF 5600

ORGANISM(S): Naja (ncbitaxon:8638)

SUBMITTER: Eivind Undheim Daniel Petras

PROVIDER: MSV000081885 | MassIVE | Thu Jan 04 17:11:00 GMT 2018

SECONDARY ACCESSION(S): PXD008597

REPOSITORIES: MassIVE

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Publications

Convergent evolution of pain-inducing defensive venom components in spitting cobras.

Kazandjian T D TD Petras D D Robinson S D SD van Thiel J J Greene H W HW Arbuckle K K Barlow A A Carter D A DA Wouters R M RM Whiteley G G Wagstaff S C SC Arias A S AS Albulescu L-O LO Plettenberg Laing A A Hall C C Heap A A Penrhyn-Lowe S S McCabe C V CV Ainsworth S S da Silva R R RR Dorrestein P C PC Richardson M K MK Gutiérrez J M JM Calvete J J JJ Harrison R A RA Vetter I I Undheim E A B EAB Wüster W W Casewell N R NR

Science (New York, N.Y.) 20210101 6527

Convergent evolution provides insights into the selective drivers underlying evolutionary change. Snake venoms, with a direct genetic basis and clearly defined functional phenotype, provide a model system for exploring the repeated evolution of adaptations. While snakes use venom primarily for predation, and venom composition often reflects diet specificity, three lineages of cobras have independently evolved the ability to spit venom at adversaries. Using gene, protein, and functional analyses, ...[more]

PMID: 33479150

Dataset Information

Genus wide venom proteomics of cobras (Naja)

Dataset's files

Publications

Convergent evolution of pain-inducing defensive venom components in spitting cobras.

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