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Quantitative top down mass spectrometry identifies proteoforms differentially secreted by mechanical stimulation of mouse skin

ABSTRACT: This study presents a first step towards identifying soluble mediators of keratinocyte-sensory neuron communication by evaluating the potential for top down mass spectrometry to identify proteoforms secreted during one minute of mechanical stimulation of mouse skin from na�ve animals. Overall, this study identified 47 proteoforms in the secretome of mouse hindpaw skin, of which 14 were deferentially secreted during mechanical stimulation, and includes proteins with known and previously unknown relevance to mechanotransduction. Finally, this study outlines a bioinformatic workflow that merges output from two complementary analysis platforms for top down data and demonstrates the utility of this workflow for integrating quantitative and qualitative data.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Rebekah Gundry

PROVIDER: MSV000082059 | MassIVE | Mon Feb 12 14:32:00 GMT 2018

REPOSITORIES: MassIVE

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