Proteomics

Dataset Information

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Chapman_CCM_2018


ABSTRACT: This submission contains the mass spectrometry files for the manuscript by Chapman and colleagues that characterize KRI-1 in C. elegans by affinity purification experiments of KRI-1 fused to GFP. Controls used for this experiment include ACT-5 fused to GFP and N2 worms. This dataset consists of 18 raw MS files and associated with peak lists (mzML) and mass spectrometry results (mzid) in standard formats, acquired on a Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap operated in DDA mode. Samples were generated by Eric Chapman and affinity purification, mass spectrometric acquisition and analysis was performed by Christopher Go. Raw files are deposited here in MassIVE. Brent Derry is the corresponding author of the manuscript (brent.derry@sickkids.ca); Anne-Claude Gingras should be contacted for questions on this dataset (gingras@lunenfeld.ca).

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Caenorhabditis Elegans (ncbitaxon:6239)

SUBMITTER: Anne-Claude Gingras  

PROVIDER: MSV000082400 | MassIVE | Thu May 24 11:17:00 BST 2018

SECONDARY ACCESSION(S): PXD009886

REPOSITORIES: MassIVE

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Publications


Apoptotic death of cells damaged by genotoxic stress requires regulatory input from surrounding tissues. The C. elegans scaffold protein KRI-1, ortholog of mammalian KRIT1/CCM1, permits DNA damage-induced apoptosis of cells in the germline by an unknown cell non-autonomous mechanism. We reveal that KRI-1 exists in a complex with CCM-2 in the intestine to negatively regulate the ERK-5/MAPK pathway. This allows the KLF-3 transcription factor to facilitate expression of the SLC39 zinc transporter g  ...[more]

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