Eicosanoid measurements from EDTA plasma from 1500 people
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Thermo QExactive Orbitrap
ORGANISM(S): Homo Sapiens
SUBMITTER: 
Mohit Jain
PROVIDER: MSV000082852 | MassIVE | Sat Aug 25 19:02:00 BST 2018
REPOSITORIES: MassIVE
Similar Datasets
Project description:Eicosanoid measurement from EDTA plasma from 1500 people. Collected in negative ion mode.
2018-08-25 | MSV000082852 | GNPS
Project description:To further understanding of mature microRNA in plasma from healthy people, we have employed microRNA microarray as a discovery platform with the potential to identify the plasma microRNA levels of healthy people. Human peripheral blood was drawn from 4 healthy donors, and plasma was obtained by two-step centrifugation. Equal of total RNA from the plasma was detected by microRNA microarray including 866 human and 89 human viral miRNAs (Sanger miRBase, release 12.0). About 170 miRNAs could be detected by microarray in all 4 samples of plasma. Among them, 6 microRNAs (miR-451, miR-16, miR-133a, miR-1, miR-499 and miR-208a) were detected in the RNA from the same plasma by real-time PCR. Freshly peripheral bloods from 4 healthy donors were drawn into EDTA tubes, and were processed within 1 hour by two-step centrifugation to remove the blood cells and the cellular debris. Small RNA was prepared using the mirVana PARIS kit, and was quantified using a NanoDrop-1000 spectrophotometer. Equal of RNA from the plasma was detected by microRNA microarray to identify the microRNAs levels in plasma of healthy people.
2010-08-03 | E-GEOD-23390 | biostudies-arrayexpress
Project description:To further understanding of mature microRNA in plasma from healthy people, we have employed microRNA microarray as a discovery platform with the potential to identify the plasma microRNA levels of healthy people. Human peripheral blood was drawn from 4 healthy donors, and plasma was obtained by two-step centrifugation. Equal of total RNA from the plasma was detected by microRNA microarray including 866 human and 89 human viral miRNAs (Sanger miRBase, release 12.0). About 170 miRNAs could be detected by microarray in all 4 samples of plasma. Among them, 6 microRNAs (miR-451, miR-16, miR-133a, miR-1, miR-499 and miR-208a) were detected in the RNA from the same plasma by real-time PCR.
2010-08-03 | GSE23390 | GEO
Project description:Advancing Negative Ion Mode Proteomics. The main objective of the project is the exploration of the unconvetional negative ion mode for proteomics studies. In this work, we thoroughly studied the best chromatographic conditions for negative ion mode proteomics before testing different enzymatic digestion. The final goal is to establish the best working conditions in the negative polarity for negative ion mode. The method also refrains from any fragmentation events, which are unpredictable in negative ion mode.
2023-07-20 | PXD040583 | Pride
Project description:Exploring differentially expressed miRNAs (DEmiRNAs) in plasma sample between lung adenocarcinoma patients and healthy people using a small RNA (sRNA) sequencing,results showed that we could used these DEmiRNAs identified could discriminate healthy peoples from lung adenocarcinoma patients. In present study, we applied an RNA sequencing (RNA-seq) approach to explore the differentially expressed miRNAs (DEmiRNAs) in plasma sample between 6 lung adenocarcinoma patients and 4 healthy people.
2021-03-09 | GSE151963 | GEO
Project description:Elite controllers maintain HIV-1 viral loads below the limit of detection. The mechanisms responsible for this phenomenon are poorly understood. As microRNAs (miRNAs) are regulators of gene expression and some of them modulate HIV infection, we have studied the miRNA profile in plasma from HIV elite controllers and chronically infected individuals and compared against healthy donors. Several miRNAs correlate with CD4+ T cell count or with the known time of infection. No significant differences were observed between elite controllers and healthy donors; however, 16 miRNAs were different in the plasma of chronic infected versus healthy donors. In addition, levels of hsa-miR-29b-3p, hsa-miR-33a-5p and hsa-miR-146a-5p were higher in plasma from elite controllers than chronic infected and hsa-miR-29b-3p and hsa-miR-33a-5p overexpression significantly reduced the viral production in MT2 cells. Therefore, levels of circulating miRNAs might be of diagnostic and/or prognostic value for HIV infection. Additionally, hsa-miR-29b-3p and miR-33a-5p may be used in therapeutic strategies. An exploratory cross-sectional study of microRNA levels in EDTA plasma samples. Plasma samples were obtained from 24 subjects and were classified in 3 groups, 9 Elite Controllers (defined as individuals with plasma viral load (PVL) < 50 copies/ml, CD4 count >350/ml), 9 chronic HIV patients (CH) under anti-retroviral treatment and 6 healthy HIV negative donors (HD). This study was approved by the HuM-CM-)sped Foundation Ethics Committee and informed consent was obtained from all subjects.
2014-08-04 | E-GEOD-58868 | biostudies-arrayexpress
Project description:Trauma patients vs Normals, EDTA-plasma, multipeptide proteins and N-glycoproteins
2009-05-15 | PRD000073 | Pride
Project description:We seek to discover small RNA biomarkers of autoimmune activity andor beta cell damage in type 1 diabetes. Pilot studies showed that heparinized plasma failed analyses, but that EDTA and citrated plasma did well, so 353 appropriate plasma samples average 11 per subject prospectively collected every 1 to 3 months from 32 high risk MAB or newly diabetic children and adolescents were collected, and the first 94 analyzed, for circulating small regulatory RNAs. 92 of 94 resulting cDNA libraries gave adequate numbers of miRNA mapped reads, but QC using spiked RNA internal standards showed abnormally high small RNA levels in 8 mildly hemolyzed plasma samples, leaving 84 of 94 with analyzable data. Equal numbers of EDTA and citrated plasma were analyzed successfully. Over the next period we will complete series on another 6 subjects, sequence the remaining 26070340 samples, and analyze the data for patterns of disease association with small RNA molecules in the prediabetic, perionset, and immediate post onset period. These patterns may identify biomarker small RNA predictive of autoimmune flares or beta cell loss, including predicting impending clinical onset.
2022-10-30 | GSE216551 | GEO
Project description:Pooled samples from human plasma of groups of people with normal intestine, IBD, colorectal adenoma and cancer
| PRJEB1731 | ENA
Project description:Over the last two decades, EDTA-plasma has been used as the preferred sample matrix for human blood proteomic profiling. Serum has also been employed widely. Only a few studies have assessed the difference and relevance of the proteome profiles obtained from plasma samples, such as EDTA-plasma or lithium-heparin-plasma, and serum. A complete evaluation of the use of EDTA-plasma, heparin-plasma, and serum would greatly expand the comprehensiveness of shotgun proteomics of blood samples. In this study, we evaluated the use of heparin-plasma with respect to EDTA-plasma and serum to profile blood proteomes using a scalable automated proteomic pipeline (ASAP2). The use of plasma and serum for mass spectrometry-based shotgun proteomics was tested with commercial pooled samples. The proteome coverage consistency and the quantitative performance were compared.
2018-02-27 | PXD007846 | Pride