Proteomics of Pseudomonas aeruginosa PAO1 and PA14

ABSTRACT: Recent studies have shown that the concentrations of proteins expressed from orthologous genes are often conserved across organisms, and to a greater extent than the abundances of the corresponding mRNAs. However, such studies have not distinguished between evolutionary (e.g., sequence divergence) and environmental (e.g., growth condition) effects on the regulation of steady-state protein and mRNA abundances. Here we systematically investigated the transcriptome and proteome of two closely related Pseudomonas aeruginosa strains, PAO1 and PA14, under identical experimental conditions, thus controlling for environmental effects. For 703 genes observed by both shotgun proteomics and microarray experiments, we find that the protein-to-mRNA ratios are highly correlated between orthologous genes in the two strains, to an extent comparable to protein and mRNA abundances. In spite of this high molecular similarity between PAO1 and PA14, we found that several metabolic, virulence, and antibiotic resistance genes are differentially expressed between the two strains, mostly at the protein but not at the mRNA level. Our data demonstrate that post-transcriptional regulation is important for understanding the discordance between mRNA and protein abundance.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Pseudomonas Aeruginosa Pa14 (ncbitaxon:652611) Pseudomonas Aeruginosa Pao1 (ncbitaxon:208964)

SUBMITTER: Edward Marcotte  

PROVIDER: MSV000082863 | MassIVE | Wed Aug 29 12:53:00 BST 2018

SECONDARY ACCESSION(S): PXD000684

REPOSITORIES: MassIVE