Dataset Information

Cytosolic proteome of Staphylococcus aureus HG001 post internalization in bronchial epithelial cells - S. aureus dataset

ABSTRACT: The data set consists of 32 S. aureus cytosolic samples that were collected by GFP supported cell sorting during infection in lung epithelial cells. The data include 8 time points (2.5 h, 6.5 h , 24 h, 48 h, 72 h, and 96 h post infection plus exponential and non-adherent control) that were collected out of four biological replicates. DIA data are provided. The respective spectral library was already published (Michalik et al., 2017, Sci Rep. doi: 10.1038/s41598-017-10059-w.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Staphylococcus Aureus Hg001

SUBMITTER: Prof. Dr. Uwe Voelker

PROVIDER: MSV000082920 | MassIVE |

SECONDARY ACCESSION(S): PXD011028

REPOSITORIES: MassIVE

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Publications

Metabolic Cross-talk Between Human Bronchial Epithelial Cells and Internalized <i>Staphylococcus aureus</i> as a Driver for Infection.

Palma Medina Laura M LM Becker Ann-Kristin AK Michalik Stephan S Yedavally Harita H Raineri Elisa J M EJM Hildebrandt Petra P Gesell Salazar Manuela M Surmann Kristin K Pförtner Henrike H Mekonnen Solomon A SA Salvati Anna A Kaderali Lars L van Dijl Jan Maarten JM Völker Uwe U

Molecular & cellular proteomics : MCP 20190226 5

<i>Staphylococcus aureus</i> is infamous for causing recurrent infections of the human respiratory tract. This is a consequence of its ability to adapt to different niches, including the intracellular milieu of lung epithelial cells. To understand the dynamic interplay between epithelial cells and the intracellular pathogen, we dissected their interactions over 4 days by mass spectrometry. Additionally, we investigated the dynamics of infection through live cell imaging, immunofluorescence and e ...[more]

PMID: 30808728

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Project description:Staphylococcus aureus is a human pathogen that can cause a wide range of diseases. Although formerly regarded as extracellular pathogen, it has been shown that S. aureus can also be internalized by host cells and persist within these cells. In the present study, we comparatively analyzed survival and physiological adaptation of S. aureus HG001 after internalization by two human lung epithelial cell lines (S9 and A549), and human embryonic kidney cells (HEK 293). Combining enrichment of bacteria from host-pathogen assays by cell sorting and quantitation of the pathogen's proteome by mass spectrometry we characterized S. aureus adaptation during the initial phase between 2.5 h and 6.5 h post-infection. Starting with about 2 × 10^6 bacteria, roughly 1450 S. aureus proteins, including virulence factors and metabolic enzymes were identified by spectral comparison and classical database searches. Most of the bacterial adaptation reactions, such as decreased levels of ribosomal proteins and metabolic enzymes or increased amounts of proteins involved in arginine and lysine biosynthesis, enzymes coding for terminal oxidases and stress responsive proteins or activation of the sigma factor SigB were observed after internalization into any of the three cell lines studied. However, differences were noted in central carbon metabolism including regulation of fermentation and threonine degradation. Since these differences coincided with different intracellular growth behavior, complementary profiling of the metabolome of the different non-infected host cell types was performed. This revealed similar levels of intracellular glucose but host cell specific differences in the amounts of amino acids such as glycine, threonine or glutamate. With this comparative study we provide an impression of the common and specific features of the adaptation of S. aureus HG001 to specific host cell environments as a starting point for follow-up studies with different strain isolates and regulatory mutants. Metabolome analysis, by nuclear magnetic resonance spectroscopy, was performed for the infection medium (extracellular medium, mock infection of 1 h).