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Identification of peptides and proteins in insect based feed ingredients using LCMSMS

ABSTRACT: Identification of peptides and proteins of 18 different insect meal samples derived from the species Hermetia illucens (8), Tenebrio molitor (5), Alphitobius diaperinus (3) and Acheta domesticus (2). Proteins were extracted using two different extraction and solubilization methods. Protein digests were analyzed using an ESI-MS/MS maXis Impact UHR-TOF (Bruker) coupled with an UltiMate 3000 HPLC system (Thermo Scientific) using a 1 mm I.D. x 150 mm reverse phase column (Acclaim PepMap 100 C18, Thermo). Mass spectrometry data (.baf files) were converted using compassXport (Bruker DataAnalysis 4.2) and saved as mzXML files. Proteomics data analysis was conducted using the proteoQC package (version 1.18.1) in R (version 3.4.4) running in RStudio (version 1.0.143).

INSTRUMENT(S): maXis

ORGANISM(S): Alphitobius Diaperinus (ncbitaxon:27448) Hermetia Illucens (ncbitaxon:343691) Acheta Domesticus (ncbitaxon:6997) Tenebrio Molitor (ncbitaxon:7067)

SUBMITTER: Josef Daniel Rasinger

PROVIDER: MSV000083737 | MassIVE | Wed May 01 20:05:00 BST 2019

REPOSITORIES: MassIVE

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Identification of peptides and proteins in insect based feed ingredients using LCMSMS

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