Proteomics

Dataset Information

0

Site-specific analysis of CD16a and CD32a N-glycosylation from monocytes display donor variability which modulate affinity to IgG1 Fc


ABSTRACT: Glycopeptide mass spectra of CD16a and CD32a from isolated monocytes display donor and site variability. CD16a N-glycosylation composition affects the affinity of IgG1 Fc. Peptide coverage of CD16a was ~85% while CD32a was ~61%.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Adam Barb  

PROVIDER: MSV000084012 | MassIVE | Wed Jun 26 10:33:00 BST 2019

REPOSITORIES: MassIVE

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1
altmetric image

Publications

Site-specific N-glycan Analysis of Antibody-binding Fc γ Receptors from Primary Human Monocytes.

Roberts Jacob T JT   Patel Kashyap R KR   Barb Adam W AW  

Molecular & cellular proteomics : MCP 20191230 2


FcγRIIIa (CD16a) and FcγRIIa (CD32a) on monocytes are essential for proper effector functions including antibody dependent cellular cytotoxicity (ADCC) and phagocytosis (ADCP). Indeed, therapeutic monoclonal antibodies (mAbs) that bind FcγRs with greater affinity exhibit greater efficacy. Furthermore, post-translational modification impacts antibody binding affinity, most notably the composition of the asparagine(N)-linked glycan at N162 of CD16a. CD16a is widely recognized as the key receptor f  ...[more]

Similar Datasets

2017-07-03 | GSE83436 | GEO
2023-06-15 | PXD043008 | JPOST Repository
2023-12-11 | PXD047089 | Pride
| S-EPMC3365978 | biostudies-literature
2019-06-04 | MSV000083890 | MassIVE
2013-10-31 | E-GEOD-49033 | biostudies-arrayexpress
2019-01-24 | GSE109573 | GEO
2022-12-12 | GSE190421 | GEO
2020-05-26 | GSE129616 | GEO
2023-11-16 | GSE208235 | GEO