Combining state-of-the-art tandem mass spectrometry with de novo-assisted database peptide sequencing is a viable strategy for the discovery of small signaling peptides at the plant-microbe interface

ABSTRACT: Small polypeptides (sPEPs) less than 100 amino acids in length are emerging as key signaling molecules that mediate cell-cell communication and biological processes that occur within and between plants, fungi, and bacteria. In this study, we benchmarked the qualitative and quantitative performance of a 10 kDa MWCO filter enrichment strategy optimized specifically for sPEPs using shotgun proteomics with de novo-assisted database searches. We implemented this approach to identify unknown sPEPs from different tissues of Populus X canescens growing with or without Laccaria bicolor ectomycorrhiza. In total, 1660 and 2870 Populus and L. bicolor unique sPEPs were identified, respectively. By means of relative quantification, 129 sPEPs were determined to be significantly more abundant in Populus root with established ectomycorrhizae (ECM) when compared to free-living mycelium of L. bicolor. Several of these peptides mapped to proteins already implicated in biologically relevant associations between plant and fungus.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Populus Sp. (ncbitaxon:3697)

SUBMITTER: Paul Abraham  

PROVIDER: MSV000084471 | MassIVE | Fri Oct 18 05:35:00 BST 2019

SECONDARY ACCESSION(S): PXD015895

REPOSITORIES: MassIVE