Proteomics

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Combining proximity labeling and crosslinking mass spectrometry for proteomic dissection of nuclear envelope interactome

ABSTRACT: Analysis of LAP1 interactome in HEK293T cells using proximity labeling or proximity labeling combined with crosslinking methods.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Yun Mou  

PROVIDER: MSV000084606 | MassIVE | Wed Nov 20 18:22:00 GMT 2019

SECONDARY ACCESSION(S): PXD016371

REPOSITORIES: MassIVE

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Combining Proximity Labeling and Cross-Linking Mass Spectrometry for Proteomic Dissection of Nuclear Envelope Interactome.

Liu Cheng-Hao CH   Chien Ming-Jou MJ   Chang You-Chiun YC   Cheng Yu-Hsiang YH   Li Fu-An FA   Mou Kurt Yun KY  

Journal of proteome research 20200207 3

Proximity labeling (PL) and chemical cross-linking (XL) mass spectrometry are two powerful methods to dissect protein-protein interactions (PPIs) in cells. Although PL typically captures neighboring proteins within a range of 10-20 nm of a single bait protein, chemical XL defines direct protein-protein contacts within 1 nm in a systemic manner. Here, we develop a new method, named PL/XL-MS, to harness the advantages of both PL and XL. PL/XL-MS can enrich a subcellular compartment by PL and simul  ...[more]

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