Proteomics

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Identification of PIM1 substrates reveals a role for NDRG1 in prostate cancer cellular migration and invasion


ABSTRACT: PIM1 is an oncogenic serine/threonine kinase that promotes and maintains prostate tumorigenesis. To more fully understand the mechanism by which PIM1 promotes oncogenesis, we performed a chemical genetic screen to identify direct PIM1 substrates in prostate cancer cells. To identify PIM1 substrates and their phosphorylation sites in LNCaP cells, we coupled a chemical genetic screen with a peptide capture, mass spectrometry (MS)-based approach. We mutated the PIM1 gatekeeper residue in the ATP binding site to accept a bulky ATP analog. By using an ATP analog labeled with a thiol group on the gamma-phosphate, we were able specifically label PIM1 substrates even in the presence of other cellular kinases.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Beatrix Ueberheide  

PROVIDER: MSV000085028 | MassIVE | Thu Feb 27 10:04:00 GMT 2020

REPOSITORIES: MassIVE

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