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Proteomic Analysis of Lyme Disease: Global Protein Comparison of Three Strains of Borrelia Burgdorferi

ABSTRACT: We report the proteomic analysis of the archetype B. burgdorferi B31 strain and two other strains (ND40, and JD-1) having different Borrelia pathotypes using strong cation exchange fractionation of proteolytic peptides followed by high-resolution, reversed phase capillary liquid chromatography coupled with ion trap tandem mass spectrometric analysis.

INSTRUMENT(S): LCQ Classic

ORGANISM(S): Borreliella Burgdorferi (ncbitaxon:139)

SUBMITTER: Jon M. Jacobs

PROVIDER: MSV000085503 | MassIVE | Fri May 29 11:47:00 BST 2020

REPOSITORIES: MassIVE

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Proteomic analysis of Lyme disease: global protein comparison of three strains of Borrelia burgdorferi.

Jacobs Jon M JM Yang Xiaohua X Luft Benjamin J BJ Dunn John J JJ Camp David G DG Smith Richard D RD

Proteomics 20050401 5

The Borrelia burgdorferi spirochete is the causative agent of Lyme disease, the most common tick-borne disease in the United States. It has been studied extensively to help understand its pathogenicity of infection and how it can persist in different mammalian hosts. We report the proteomic analysis of the archetype B. burgdorferi B31 strain and two other strains (ND40, and JD-1) having different Borrelia pathotypes using strong cation exchange fractionation of proteolytic peptides followed by h ...[more]

PMID: 15800874

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