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Cathepsin B MSP-MS at pH 4.6 and 7.2 without inhibitor

ABSTRACT: MSP-MS of Cathepsin B at pH 4.6 and pH 7.2 without inhibitor

INSTRUMENT(S): Q Exactive

ORGANISM(S): Synthetic Construct (ncbitaxon:32630)

SUBMITTER: Vivian Hook

PROVIDER: MSV000086449 | MassIVE | Thu Nov 12 16:28:00 GMT 2020

SECONDARY ACCESSION(S): PXD022494

REPOSITORIES: MassIVE

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Publications

Molecular Features of CA-074 pH-Dependent Inhibition of Cathepsin B.

Yoon Michael C MC Christy Mitchell P MP Phan Von V VV Gerwick William H WH Hook Gregory G O'Donoghue Anthony J AJ Hook Vivian V

Biochemistry 20220204 4

CA-074 is a selective inhibitor of cathepsin B, a lysosomal cysteine protease. CA-074 has been utilized in numerous studies to demonstrate the role of this protease in cellular and physiological functions. Cathepsin B in numerous human disease mechanisms involves its translocation from acidic lysosomes of pH 4.6 to neutral pH 7.2 of cellular locations, including the cytosol and extracellular environment. To gain in-depth knowledge of CA-074 inhibition under these different pH conditions, this st ...[more]

PMID: 35119840

Publication: 1/2

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