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MSP-MS of Cathepsin B and Cathepsin K pH 4.6 pH 7.2

ABSTRACT: MSP-MS of Cathepsin B and Cathepsin K pH 4.6 pH 7.2

INSTRUMENT(S): Q Exactive

ORGANISM(S): Synthetic Construct (ncbitaxon:32630)

SUBMITTER: Vivian Hook Anthony O'Donoghue

PROVIDER: MSV000088932 | MassIVE | Mon Feb 28 18:39:00 GMT 2022

SECONDARY ACCESSION(S): PXD031957

REPOSITORIES: MassIVE

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Publications

Cathepsin B Dipeptidyl Carboxypeptidase and Endopeptidase Activities Demonstrated across a Broad pH Range.

Yoon Michael C MC Hook Vivian V O'Donoghue Anthony J AJ

Biochemistry 20220818 17

Cathepsin B is a lysosomal protease that participates in protein degradation. However, cathepsin B is also active under neutral pH conditions of the cytosol, nuclei, and extracellular locations. The dipeptidyl carboxypeptidase (DPCP) activity of cathepsin B, assayed with the Abz-GIVR↓AK(Dnp)-OH substrate, has been reported to display an acidic pH optimum. In contrast, the endopeptidase activity, monitored with Z-RR-↓AMC, has a neutral pH optimum. These observations raise the question of whether ...[more]

PMID: 35981509

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