Dataset Information

Proteomic and Genomic Methylation Signatures of Idiopathic Subglottic Stenosis

ABSTRACT: LC-MS/MS data obtained from subglottic biopsies collected from 12 individuals with idiopathic subglottic stenosis, as well as 3 age-, sex-, and race/ethnicity-matched controls. All tissue donors were women of White race and non-Latino or Hispanic ethnicity. Sample preparation, mass spectrometry, and data analysis details are available in the published article.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Nathan Welham

PROVIDER: MSV000086498 | MassIVE | Sat Nov 21 15:09:00 GMT 2020

REPOSITORIES: MassIVE

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Proteomic and Genomic Methylation Signatures of Idiopathic Subglottic Stenosis.

Schoeff Stephen S SS Shi Xudong X Young William G WG Whited Chad W CW Soni Resha S RS Liu Peng P Ong Irene M IM Dailey Seth H SH Welham Nathan V NV

The Laryngoscope 20200703 2

<h4>Objective</h4>Idiopathic subglottic stenosis (iSGS) is a chronic inflammatory condition that causes dyspnea and affects middle-aged women of White race and non-Latino or Hispanic ethnicity. To better characterize its phenotype and pathogenesis, we assessed the proteomic and genomic methylation signatures of subglottic tissue collected from iSGS patients compared to controls.<h4>Study design</h4>Molecular analysis of clinical biospecimens.<h4>Methods</h4>We collected subglottic tissue biopsie ...[more]

PMID: 32619300

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Project description:ObjectiveIdiopathic subglottic stenosis (iSGS) is a chronic inflammatory condition that causes dyspnea and affects middle-aged women of White race and non-Latino or Hispanic ethnicity. To better characterize its phenotype and pathogenesis, we assessed the proteomic and genomic methylation signatures of subglottic tissue collected from iSGS patients compared to controls.Study designMolecular analysis of clinical biospecimens.MethodsWe collected subglottic tissue biopsies from 12 patients during direct laryngoscopy, immediately prior to surgical treatment of iSGS; as well as from 4 age-, sex-, and race/ethnicity-matched control patients undergoing other direct laryngoscopic procedures. We isolated protein and genomic DNA, acquired proteomic data using label-free quantitative mass spectrometry techniques, and acquired genome-wide methylation data using bisulfite conversion and a microarray platform. We compared molecular profiles across the iSGS and control groups, and with respect to clinical course in the iSGS group. Eight of the 12 iSGS patients underwent subsequent blood collection and plasma isolation for further assessment.ResultsProteomic analysis revealed 42 differentially abundant proteins in the iSGS biopsies compared to controls, inferring enrichment of biological pathways associated with early wound healing, innate immunity, matrix remodeling, and metabolism. Proteome-based hierarchical clustering organized patients into two iSGS and one control subgroups. Methylation analysis revealed five hypermethylated genes in the iSGS biopsies compared to controls, including the biotin recycling enzyme biotinidase (BTD). Follow-up analysis showed elevated plasma BTD activity in iSGS patients compared to both controls and published normative data.ConclusioniSGS exhibits distinct proteomic and genomic methylation signatures. These signatures expand current understanding of the iSGS phenotype, support the possibility of disease subgroups, and should inform the direction of future experimental studies.Level of evidenceNot applicable Laryngoscope, 131:E540-E546, 2021.