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Skeletal Muscle Metabolism of the Astyanax mexicanus

ABSTRACT: The muscle composition of cave dwelling and surface fish was analyzed by proteomics. Specifically, the composition of the myosin heavy chain was determined by in-gel digestion followed by reversed phase LC-MS/MS. The proteome of the different muscle types was analyzed by tandem mass tags and phosphorylation differences were determined by enriching for the modification.

INSTRUMENT(S): Orbitrap Eclipse, LTQ Orbitrap Velos

ORGANISM(S): Astyanax Mexicanus (ncbitaxon:7994)

SUBMITTER: Laurence Florens

PROVIDER: MSV000086857 | MassIVE | Fri Feb 12 07:49:00 GMT 2021

SECONDARY ACCESSION(S): PXD024165

REPOSITORIES: MassIVE

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Publications

Metabolic reprogramming underlies cavefish muscular endurance despite loss of muscle mass and contractility.

Olsen Luke L Levy Michaella M Medley J Kyle JK Hassan Huzaifa H Miller Brandon B Alexander Richard R Wilcock Emma E Yi Kexi K Florens Laurence L Weaver Kyle K McKinney Sean A SA Peuß Robert R Persons Jenna J Kenzior Alexander A Maldonado Ernesto E Delventhal Kym K Gluesenkamp Andrew A Mager Edward E Coughlin David D Rohner Nicolas N

Proceedings of the National Academy of Sciences of the United States of America 20230124 5

Physical inactivity is a scourge to human health, promoting metabolic disease and muscle wasting. Interestingly, multiple ecological niches have relaxed investment into physical activity, providing an evolutionary perspective into the effect of adaptive physical inactivity on tissue homeostasis. One such example, the Mexican cavefish <i>Astyanax mexicanus,</i> has lost moderate-to-vigorous activity following cave colonization, reaching basal swim speeds ~3.7-fold slower than their river-dwelling ...[more]

PMID: 36693105

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The effect of HDAC inhibitor, 4b, on skeletal muscle gene expression

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MicroRNA expression profiling of skeletal muscle from PPARalpha- and PPARbeta-overexpressing mice

Project description:This experiment was conducted to identify target microRNAs of the peroxisome proliferator-activated receptor (PPAR) in skeletal muscle of transgenic mice that overexpressed PPARalpha or PPARbeta. We have recently demonstrated that skeletal muscle-specific PPARb transgenic (MCK-PPARb) mice exhibit increased exercise endurance, whereas MCK-PPARa mice have reduced exercise performance. Accordingly, we sought to determine whether PPARb and PPARa drive distinct programs involved in muscle fiber type determination. Myosin heavy chain (MHC) immunohistochemical staining of soleus muscle revealed a marked increase in type 1 fibers in the MCK-PPARb muscle compared to non-transgenic (NTG) littermates but a profound reduction in MCK-PPARa muscle. miRNA profiling revealed that levels of miR-208b and miR-499 were increased in MCK-PPARb muscle but reduced in MCK-PPARa muscle. miR-208b and miR-499, which are embedded in the Myh7 and Myh7b genes, respectively, have been shown previously to regulate slow-twitch muscle genes. Lastly, combined inhibition of miR-208b and miR-499 abolished the enhancing effects of PPARb on MHC1 expression in skeletal myotubes, while forced expression of miR-499 in MCK-PPARa muscle completely reversed the type 1 fiber program and exercise capacity. Taken together, these findings demonstrate that miR-208b and miR-499 are necessary to mediate the effects of PPARb and PPARa on muscle fiber type determination. Comparison of microRNA expression from soleus muscles isolated from wild-type (non-transgenic (NTG)) and PPARalpha-overexpressing (MCK-PPARa) mice, and comparison of microRNA expression from soleus muscles isolated from wild-type (NTG) and PPARbeta-overexpressing (MCK-PPARb) mice. Three replicates of each are analyzed.

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