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Proteomic analysis of skeletal muscle fibre samples from hibernating and awake bears

ABSTRACT: Recent discoveries in the field of skeletal muscle have found that the contractile protein myosin is able to dictate the resting metabolic rate of this organ. In this study we aimed to investigate if myosin is involved in the process of metabolic shutdown which is observed during hibernation. We found that in small hibernating mammals, the rate of ATP turnover dictated by myosin was altered and that this was also dependent upon the temperature of skeletal muscle. We further investigated changes to the whole proteome of these animals and observed that in small hibernators found significant changes to sarcomere organization during hibernating periods. Finally, we identified hyperphosphorylation upon the myosin molecule in these small hibernators which was predicted to induce stability changes to this molecule.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Ursus Arctos Arctos (european Brown Bear)

TISSUE(S): Skeletal Muscle Fiber

SUBMITTER: Michael Wierer

LAB HEAD: Julien Ochala

PROVIDER: PXD044505 | Pride | 2024-04-29

REPOSITORIES: Pride

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			Action	DRS
	20230502_ASC2_NEO3_PRI_P79_R0718_120min_uPAC110cm_TMTpro_MS3_Fr1.raw	Raw
	20230502_ASC2_NEO3_PRI_P79_R0718_120min_uPAC110cm_TMTpro_MS3_Fr10.raw	Raw
	20230502_ASC2_NEO3_PRI_P79_R0718_120min_uPAC110cm_TMTpro_MS3_Fr11.raw	Raw
	20230502_ASC2_NEO3_PRI_P79_R0718_120min_uPAC110cm_TMTpro_MS3_Fr12.raw	Raw
	20230502_ASC2_NEO3_PRI_P79_R0718_120min_uPAC110cm_TMTpro_MS3_Fr13.raw	Raw

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