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Proteomic analysis of skeletal muscle fibre samples from hibernating and awake squirrels

ABSTRACT: Recent discoveries in the field of skeletal muscle have found that the contractile protein myosin is able to dictate the resting metabolic rate of this organ. In this study we aimed to investigate if myosin is involved in the process of metabolic shutdown which is observed during hibernation. Comparing large and small hibernating mammals, we found that in small hibernating mammals, the rate of ATP turnover dictated by myosin was altered and that this was also dependent upon the temperature of skeletal muscle. We further investigated changes to the whole proteome of these animals and observed that in small hibernators found significant changes to sarcomere organization during hibernating periods. Finally, we identified hyperphosphorylation upon the myosin molecule in these small hibernators which was predicted to induce stability changes to this molecule. In this dataset, we compared the proteome of skeletal muscle fibre samples from squirrels collected in summer and winter time.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Spermophilus Tridecemlineatus (thirteen-lined Ground Squirrel)

TISSUE(S): Skeletal Muscle Fiber

SUBMITTER: Michael Wierer

LAB HEAD: Julien Ochala

PROVIDER: PXD044728 | Pride | 2024-04-29

REPOSITORIES: Pride

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			Action	DRS
	20230504_ASC2_NEO3_PRI_P79_R0720_120min_uPAC110cm_TMTpro_MS3_Fr1.raw	Raw
	20230504_ASC2_NEO3_PRI_P79_R0720_120min_uPAC110cm_TMTpro_MS3_Fr10.raw	Raw
	20230504_ASC2_NEO3_PRI_P79_R0720_120min_uPAC110cm_TMTpro_MS3_Fr11.raw	Raw
	20230504_ASC2_NEO3_PRI_P79_R0720_120min_uPAC110cm_TMTpro_MS3_Fr12.raw	Raw
	20230504_ASC2_NEO3_PRI_P79_R0720_120min_uPAC110cm_TMTpro_MS3_Fr13.raw	Raw

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Proteomic analysis of skeletal muscle fibre samples from hibernating and awake bears

Project description:Recent discoveries in the field of skeletal muscle have found that the contractile protein myosin is able to dictate the resting metabolic rate of this organ. In this study we aimed to investigate if myosin is involved in the process of metabolic shutdown which is observed during hibernation. We found that in small hibernating mammals, the rate of ATP turnover dictated by myosin was altered and that this was also dependent upon the temperature of skeletal muscle. We further investigated changes to the whole proteome of these animals and observed that in small hibernators found significant changes to sarcomere organization during hibernating periods. Finally, we identified hyperphosphorylation upon the myosin molecule in these small hibernators which was predicted to induce stability changes to this molecule.

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Project description:Recent discoveries in the field of skeletal muscle have found that the contractile protein myosin is able to dictate the resting metabolic rate of this organ. In this study we aimed to investigate if myosin is involved in the process of metabolic shutdown which is observed during hibernation. Comparing large and small hibernating mammals, we found that in small hibernating mammals, the rate of ATP turnover dictated by myosin was altered and that this was also dependent upon the temperature of skeletal muscle. We further investigated changes to the whole proteome of these animals and observed that in small hibernators found significant changes to sarcomere organization during hibernating periods. Finally, we identified hyperphosphorylation upon the myosin molecule in these small hibernators which was predicted to induce stability changes to this molecule. In this dataset, we performed PTM peptide mapping of the myosin protein derived from skeletal muscle fibre samples from squirrels collected in summer and winter time.

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Gene Expression Data of Hibernating and Summer Active Black Bears

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