Effective Enrichment Strategy Using Boronic Acid Functionalized Mesoporous Graphene Silica Composites for Intact Glycopeptide Analysis in Human Serum

ABSTRACT: The heterogeneity and low abundance of protein glycosylation present challenging barriers to the analysis of intact glycopeptides, which is key to comprehensively understanding the role of glycosylation in an organism. Efficient and specific enrichment of intact glycopeptides could help greatly with this problem. Here, we propose a new enrichment strategy using a boronic acid functionalized mesoporous graphene silica composite for isolating intact glycopeptides from complex biological samples. The merits of this composite, including high surface area and synergistic effect from size exclusion functionality of mesoporous material, hydrophilic interaction of silica, and the reversible covalent binding with BA, enable the effective and specific enrichment of both intact glycopeptides. The results from the enrichment performance of the strategy evaluated by standard glycoproteins and the application to global glycosylation analyses in human serum indicate the robustness and potential of the strategy for intact glycopeptide analysis.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Human

SUBMITTER: Weiqian Cao  

PROVIDER: MSV000087324 | MassIVE | Wed Apr 28 02:31:00 BST 2021

REPOSITORIES: MassIVE