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Drone honey bees are disproportionately sensitive to abiotic stressors relative to workers, despite expressing high levels of stress response proteins

ABSTRACT: Drone honey bees are the obligate sexual partners of queens, and the availability of healthy, high-quality drones directly affects the queen's fertility and the productivity of her subsequent colony. Yet, our understanding of how stressors affect drone fertility and physiology is presently limited. Like other male Hymenopterans, drones are haploid and are thus expected to be more sensitive to stressors than workers, as suggested by the haploid susceptibility hypothesis. We used quantitative proteomics to investigate protein expression profiles in the hemolymph of topically exposed workers and drones, and we show that drones express surprisingly high levels of putative stress response proteins - even in the negative control groups - relative to workers. These findings suggest that drones may invest in strong constitutive expression of damage-mitigating proteins for a wide range of stressors, potentially to the detriment of launching a more specific response to new stressors. Regardless of the underlying mechanism, the robust expression patterns of proteins involved in stress responses in drones suggests that drone stress tolerance systems are fundamentally rewired relative to workers, and their susceptibility to stress depends on more than simply gene dose or deleterious recessive alleles.

INSTRUMENT(S): Bruker Impact II QTOF, Bruker TIMS TOF

ORGANISM(S): Apis Mellifera (ncbitaxon:7460)

SUBMITTER: Leonard Foster

PROVIDER: MSV000087818 | MassIVE | Wed Jul 14 10:22:00 BST 2021

SECONDARY ACCESSION(S): PXD027311

REPOSITORIES: MassIVE

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