Dataset Information

Implantation-on-a-chip cellular proteome

ABSTRACT: We have designed an implantation-on-a-chip device to model the invasion of specialized fetal extravillous trophoblasts (EVTs) from an implanting embryo into the maternal decidua. We profiled the cellular proteome of sorted EVTs or endothelial cells from devices containing different cell compositions: 1) EVTs monoculture, 2) Endothelial cells monoculture, and 3) EVTs and endothelial cells co-cultured. The proteins were extracted using the MPLEx extraction method (Nakayasu et. al. PMCID: PMC5069757), digested with 1:50 (trypsin:protein, w:w) for 3 hr at 37 C. Solid phase extraction (SPE) was performed on the samples using 1 mL/50 mg columns from Phenomenex as previously described (PMCID: PMC7192326). Peptidic concentration was assayed using a BCA assay and 5 ul at 0.1 ug/ul were injected on the LC-MS/MS system. Data was searched with MaxQuant.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Geremy Clair

PROVIDER: MSV000088677 | MassIVE | Wed Jan 12 16:10:00 GMT 2022

REPOSITORIES: MassIVE

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Publications

A microphysiological model of human trophoblast invasion during implantation.

Park Ju Young JY Mani Sneha S Clair Geremy G Olson Heather M HM Paurus Vanessa L VL Ansong Charles K CK Blundell Cassidy C Young Rachel R Kanter Jessica J Gordon Scott S Yi Alex Y AY Mainigi Monica M Huh Dan Dongeun DD

Nature communications 20220315 1

Successful establishment of pregnancy requires adhesion of an embryo to the endometrium and subsequent invasion into the maternal tissue. Abnormalities in this critical process of implantation and placentation lead to many pregnancy complications. Here we present a microenigneered system to model a complex sequence of orchestrated multicellular events that plays an essential role in early pregnancy. Our implantation-on-a-chip is capable of reconstructing the three-dimensional structural organiza ...[more]

PMID: 35292627

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