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The left ventricular phosphoproteome in mouse with cardiac-specific adenylyl cyclase type 8 overexpression

ABSTRACT: To study the molecular mechanism of adaption in the mouse with heart-specific overexpression of Adenylyl Cyclase 8 (TGAC8), we performed TMT-labeling mass spectrometry to obtain both total proteome and phosphoproteome of mouse left ventricles. Proteins were extracted from five wild-type and four TGAC8 mice and then digested with trypsin. After TMT labeling, the labeled peptides were mixed and loaded into RP-HPLC and LC-MS/MS (Thermo Orbitrap Lumos mass spectrometer). For phosphoproteome, pepetides were first enriched with TiO2 and the flow-through from TiO2 went for Fe-IMAC enrichment. The database search and statistical analysis were conducted within Proteome Discover 2.2 software.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Edward G. Lakatta

PROVIDER: MSV000089850 | MassIVE | Fri Jul 08 06:51:00 BST 2022

REPOSITORIES: MassIVE

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