Dataset Information

Proteomics of treatment of Arabidopsis thaliana with peptides

ABSTRACT: Plants were treated with a cPEP for luciferase (PEP Luc) or a scrambled version of this peptide (4 biological replicates each). Leaves were snap frozen in liquid nitrogen and grounded into powder. The powder was resuspended in tris 50 mM and 5% SDS, sonicated and centrifuged at 14,000 g for 10 min. A BCA protein assay was performed to estimate protein concentration in each sample. 50 ug of proteins were then loaded on a 12% Tris-glycine gel. Proteins were concentrated into one band and in gel digestion was performed as previously described (PMID: 25561571). 500 ng were then injected on a Thermofisher Exploris 480.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702)

SUBMITTER: Bertrand Fabre

PROVIDER: MSV000090305 | MassIVE |

REPOSITORIES: MassIVE

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Publications

Complementary peptides represent a credible alternative to agrochemicals by activating translation of targeted proteins.

Ormancey Mélanie M Guillotin Bruno B Merret Rémy R Camborde Laurent L Duboé Carine C Fabre Bertrand B Pouzet Cécile C Impens Francis F Van Haver Delphi D Carpentier Marie-Christine MC Clemente Hélène San HS Aguilar Marielle M Lauressergues Dominique D Scharff Lars B LB Pichereaux Carole C Burlet-Schiltz Odile O Bousquet-Antonelli Cécile C Gevaert Kris K Thuleau Patrice P Plaza Serge S Combier Jean-Philippe JP

Nature communications 20230117 1

The current agriculture main challenge is to maintain food production while facing multiple threats such as increasing world population, temperature increase, lack of agrochemicals due to health issues and uprising of weeds resistant to herbicides. Developing novel, alternative, and safe methods is hence of paramount importance. Here, we show that complementary peptides (cPEPs) from any gene can be designed to target specifically plant coding genes. External application of synthetic peptides inc ...[more]

PMID: 36650156

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