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Proteome wide analysis of hydrogen peroxide-induced protein carbonylation in Arabidopsis thaliana

ABSTRACT: Protein carbonylation is a non-enzymatic and irreversible post-translational modification that occurs naturally in living organisms under the direct or indirect effect of reactive oxygen species (ROS). In this study, we analyzed proteins that are responsive to carbonylation by exogenous hydrogen peroxide (H2O2) by profiling the carbonylated proteome extracted from Arabidopsis thaliana leaves after H2O2 treatment. Carbonylated proteins were enriched at the peptide level and analyzed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The results revealed that most of the carbonylated proteins identified in the H2O2-treated plant samples are related to sulphate adenylyl transferases and amidophosphoribosyl transferases involved in the immune system response, defense response, and external stimulus-response.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702)

SUBMITTER: Tagnon Missihoun

PROVIDER: MSV000090540 | MassIVE | Mon Oct 17 10:59:00 BST 2022

SECONDARY ACCESSION(S): PXD037515

REPOSITORIES: MassIVE

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Proteome-wide analysis of hydrogen peroxide-induced protein carbonylation in <i>Arabidopsis thaliana</i>.

Fangue-Yapseu Georges Yannick GY Tola Adesola Julius AJ Missihoun Tagnon D TD

Frontiers in plant science 20221205

<h4>Introduction</h4>Protein carbonylation is a non-enzymatic and irreversible post-translational modification that occurs naturally in living organisms under the direct or indirect effect of reactive oxygen species (ROS). In animals, signaling pathways involving numerous carbonylated proteins have been identified, highlighting the dual role of these molecules in ROS signal transduction. In plants, studies on phytohormone signaling (auxin, methyl jasmonate, abscisic acid) have shown that reactiv ...[more]

PMID: 36544875

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