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Segal_TMT_14-3-3_AP-MS_BioID_interactors

ABSTRACT: This dataset consists of 40 raw MS files acquired on Lumos Fusion Orbitrap mass spectrometer operated in Data Dependent Acquisition mode for TMT-10plex. The files are associated with a manuscript submitted for publication by Segal et al. that determines the protein-protein and proximity interaction landscape of 14-3-3 proteins. All samples comprise either BioID or FLAG AP-MS purifications of each of the human 14-3-3 proteins (with negative controls) expressed to the same level in HEK293 Flp-In T-REx cells. Purified samples were labeled with TMT reagents, and analyzed by mass spectrometry. 32 files correspond to the BioID dataset (fractionated; MS3), and 8 files to the AP-MS dataset (non fractionated, HCD MS2).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Human Adenovirus 5 (ncbitaxon:28285) Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Anne-Claude Gingras

PROVIDER: MSV000090795 | MassIVE | Mon Nov 28 07:29:00 GMT 2022

REPOSITORIES: MassIVE

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