Segal_TMT_14-3-3_APMS_BioID_interactors

ABSTRACT: This dataset consists of 36 raw MS files acquired on Lumos Fusion Orbitrap mass spectrometer operated in Data Dependent Acquisition mode for TMT-10plex. The files are associated with a manuscript submitted for publication by Segal et al. that determines the protein-protein and proximity interaction landscape of 14-3-3 proteins. All samples comprise either BioID or FLAG AP-MS purifications of each of the human 14-3-3 proteins (with negative controls) expressed to the same level in HEK293 Flp-In T-REx cells. Purified samples were labeled with TMT reagents, and analyzed by mass spectrometry. 32 files correspond to the BioID dataset (fractionated; MS3), and 4 files to the AP-MS dataset (non fractionated, HCD MS2). Samples were generated by Dmitri Segal and Stefan Maier and affinity purification, labeling, mass spectrometry, and data analysis were performed by Stefan Maier. Corresponding authors: Mikko Taipale ([e-mail]) and Anne-Claude Gingras ([e-mail]). Anne-Claude Gingras should be contacted for questions on this dataset.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Anne-Claude Gingras  

PROVIDER: MSV000089463 | MassIVE | Thu May 12 17:58:00 BST 2022

REPOSITORIES: MassIVE