Proteomics

Dataset Information

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Segal_14-3-3_interactors_mutants_BioID_P77_VS19


ABSTRACT: This dataset consists of 37 raw MS files and associated peak lists and result files, acquired on TripleTOF6600 mass spectrometer operated in Data Dependent Acquisition mode. The files are associated with a manuscript submitted for publication by Segal et al. that determines the protein-protein and proximity interaction landscape of 14-3-3 proteins. This particular dataset consists in files associated with three baits (14-3-3 interactors) profiled in both a wild type and a 14-3-3 binding site mutant. Each bait was profiled in biological duplicates by BioID (*using the BioID2 enzyme), alongside a total of 25 negative controls (used for analysis with SAINTexpress). See details within the README file. Samples were generated by Dmitri Segal and affinity purification and mass spectrometry were performed by Zhen-Yuan Lin with oversight from Brett Larsen and Anne-Claude Gingras. Analysis was performed by Anne-Claude Gingras and Mikko Taipale. Corresponding authors: Mikko Taipale (mikko.taipale@utoronto.ca)/lead author and Anne-Claude Gingras (gingras@lunenfeld.ca). Anne-Claude Gingras should be contacted for questions on this dataset.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Human Adenovirus 5 (ncbitaxon:28285) Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Anne-Claude Gingras  

PROVIDER: MSV000089437 | MassIVE | Sat May 07 03:24:00 BST 2022

SECONDARY ACCESSION(S): PXD033756

REPOSITORIES: MassIVE

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