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Human islets treated with alpha-difluoromethylornithine in combination with interferon-gamma and interleukin-beta

ABSTRACT: Proteomic data of human islets treated for 24h with alpha-difluoromethylornithine in combination with interferon-gamma and interleukin-beta; 6 biological replicates. Samples were digested with trypsin, then analyzed by LC-DIA-MS. Data was searched with FragPipe/DIA-NN.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Ernesto Nakayasu

PROVIDER: MSV000091431 | MassIVE | Tue Mar 07 16:53:00 GMT 2023

REPOSITORIES: MassIVE

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Publications

Inhibition of polyamine biosynthesis preserves β cell function in type 1 diabetes.

Sims Emily K EK Kulkarni Abhishek A Hull Audrey A Woerner Stephanie E SE Cabrera Susanne S Mastrandrea Lucy D LD Hammoud Batoul B Sarkar Soumyadeep S Nakayasu Ernesto S ES Mastracci Teresa L TL Perkins Susan M SM Ouyang Fangqian F Webb-Robertson Bobbie-Jo BJ Enriquez Jacob R JR Tersey Sarah A SA Evans-Molina Carmella C Long S Alice SA Blanchfield Lori L Gerner Eugene W EW Mirmira Raghavendra G RG DiMeglio Linda A LA

Cell reports. Medicine 20231101 11

In preclinical models, α-difluoromethylornithine (DFMO), an ornithine decarboxylase (ODC) inhibitor, delays the onset of type 1 diabetes (T1D) by reducing β cell stress. However, the mechanism of DFMO action and its human tolerability remain unclear. In this study, we show that mice with β cell ODC deletion are protected against toxin-induced diabetes, suggesting a cell-autonomous role of ODC during β cell stress. In a randomized controlled trial (ClinicalTrials.gov: NCT02384889) involving 41 re ...[more]

PMID: 37918404

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