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Robust and scalable fluorosequencing technology

ABSTRACT: HLA peptides from mono-allelic B-cell line using tandem mass-spectrometry We purchased the monoallelic B-cell line (B721.221), with HLA allele A*2603, directly from the International Histocompatibility Working group (Seattle, Washington). We cultured the cells and isolated HLA peptides from approximately 300 million cells as previously described (Abelin et al. 2017). We identified the isolated HLA peptides using an LC-coupled tandem mass-spectrometer (ThermoFisher, Orbitrap Fusion Lumos) and a reference dataset of a human proteome (Swissprot), applying settings from the literature for analyzing HLA peptides (Bassani-Sternberg et al. 2015) and ProteomeDiscoverer 2.3 software (Thermofisher).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Human

SUBMITTER: Jagannath Swaminathan

PROVIDER: MSV000092015 | MassIVE | Tue May 23 10:19:00 BST 2023

SECONDARY ACCESSION(S): PXD042439

REPOSITORIES: MassIVE

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