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DDA acquisition of the immunopeptidomes of C1R-B57:01 and C1R-A02:01 cell lines

ABSTRACT: Immunopeptidomes are the peptide repertoires bound by the molecules encoded by the major histocompatibility complex (MHC) (human leukocyte antigen (HLA) in humans). These HLA-peptide complexes are presented on the cell surface for immune T cell recognition. Immunopeptidomics utilizes tandem mass spectrometry (MS/MS) to identify and quantify peptides bound to HLA molecules. The dataset deposited here contains the DDA runs used to generate the spectral libraries of the HLA-bound peptides purified and isolated from C1R-B*57:01 and C1R-A*02:01 cell lines.

INSTRUMENT(S): Orbitrap Fusion, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Mohammad Shahbazy

LAB HEAD: Anthony W. Purcell

PROVIDER: PXD034429 | Pride | 2023-02-13

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	C1R-A0201_DDA_DBsearch_FDR1_speclib_fractions.xlsx	Xlsx
	C1R-B5701_DDA_DBsearch_FDR1_speclib_fractions.xlsx	Xlsx
	F120190510_PF_C1R_B5701_Library_W632_MS_pool1.raw	Raw
	F120190510_PF_C1R_B5701_Library_W632_MS_pool2.raw	Raw
	F120190510_PF_C1R_B5701_Library_W632_MS_pool3.raw	Raw

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DIA acquisition of the immunopeptidomes of C1R-B*57:01 and C1R-A*02:01 cell lines

Project description:Immunopeptidomes are the peptide repertoires bound by the molecules encoded by the major histocompatibility complex (MHC) (human leukocyte antigen (HLA) in humans). These HLA-peptide complexes are presented on the cell surface for immune T cell recognition. Immunopeptidomics utilizes tandem mass spectrometry (MS/MS) to identify and quantify peptides bound to HLA molecules. Data-independent acquisition (DIA) has emerged as a powerful strategy for deep proteome-wide profiling, but DIA application to immunopeptidomics analyses has so far seen limited use. The dataset deposited here contains the DIA data of the HLA-bound peptides purified and isolated from C1R-B*57:01 and C1R-A*02:01 cell lines.

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