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AqpZ Single Mutant and Double Mutant Cycle Native MS

ABSTRACT: Native MS analysis of single mutant and double mutant cycle experiments. See: https://www.biorxiv.org/content/10.1101/2023.09.19.558516v1

INSTRUMENT(S): Q Exactive UHMR

ORGANISM(S): Escherichia Coli (ncbitaxon:562)

SUBMITTER: Michael Marty

PROVIDER: MSV000093064 | MassIVE | Fri Oct 06 14:59:00 BST 2023

REPOSITORIES: MassIVE

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Publications

Simultaneous Native Mass Spectrometry Analysis of Single and Double Mutants To Probe Lipid Binding to Membrane Proteins.

Jayasekera Hiruni S HS Mohona Farhana Afrin FA Ewbank Megan M Marty Michael T MT

Analytical chemistry 20240610 25

Lipids are critical modulators of membrane protein structure and function. However, it is challenging to investigate the thermodynamics of protein-lipid interactions because lipids can simultaneously bind membrane proteins at different sites with different specificities. Here, we developed a native mass spectrometry (MS) approach using single and double mutants to measure the relative energetic contributions of specific residues on Aquaporin Z (AqpZ) toward cardiolipin (CL) binding. We first mut ...[more]

PMID: 38859611

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