Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

AqpZ Single Mutant and Double Mutant Cycle Native MS

ABSTRACT: Native MS analysis of single mutant and double mutant cycle experiments. See: https://www.biorxiv.org/content/10.1101/2023.09.19.558516v1

INSTRUMENT(S): Q Exactive UHMR

ORGANISM(S): Escherichia Coli (ncbitaxon:562)

SUBMITTER: Michael Marty

PROVIDER: MSV000093064 | MassIVE | Fri Oct 06 14:59:00 BST 2023

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Publications

Simultaneous Native Mass Spectrometry Analysis of Single and Double Mutants To Probe Lipid Binding to Membrane Proteins.

Jayasekera Hiruni S HS Mohona Farhana Afrin FA Ewbank Megan M Marty Michael T MT

Analytical chemistry 20240610 25

Lipids are critical modulators of membrane protein structure and function. However, it is challenging to investigate the thermodynamics of protein-lipid interactions because lipids can simultaneously bind membrane proteins at different sites with different specificities. Here, we developed a native mass spectrometry (MS) approach using single and double mutants to measure the relative energetic contributions of specific residues on Aquaporin Z (AqpZ) toward cardiolipin (CL) binding. We first mut ...[more]

PMID: 38859611

Similar Datasets

RNA-seq of AtPIN5 overexpressing tobacco BY-2 cells

Project description:This RNA-seq is a part of the paper describing the role of PIN5 in generating auxin sinks. https://www.biorxiv.org/content/10.1101/2024.01.24.576992v1

2024-03-31 | GSE260945 | GEO

Single cell RNA-seq of bioprinted human kidney organoid

Project description:These files represent single cell RNA-Seq data generated on a 10x Chromium genomics platform from bioprinted iPSC-derived human kidney organoids differentiated according to our published protocol (Takasato et al., Nature Protocols 2016). The data contains >2000 cells that passed our QC. Data was used to confirm that bioprinted organoids contain a similar cellular composition as standard manually produced organoids - See https://www.biorxiv.org/content/10.1101/505396v1.

2020-09-01 | GSE138835 | GEO

Differential RNA abundance between S. cerevisiae yeast BY4741 strains with YAK1_WT and YAK1_Q578*

Project description:Identification of the gene differentially expressed with and without a non-sense mutation in YAK1 gene. This analysis provided a better understanding of the mechanism underlying variants affecting differently mRNA and protein abundance of genes in TRANS. Data used in the preprint: https://www.biorxiv.org/content/10.1101/2020.07.02.185413v1

2020-08-11 | GSE155998 | GEO

Minimally invasive classification of pediatric solid tumors using reduced representation bisulfite sequencing of cell-free DNA: a proof-of-principle study

Project description:This submission contains the data from https://www.biorxiv.org/content/10.1101/795047v2.full This dataset contains processed data of cell-free reduced representation bisulfite sequencing from 60 pediatric cancer samples, in order to classify them according to histopathological diagnosis. Files are provided in bismark coverage format. Samples were sequenced on a NextSeq 500.

2020-02-27 | E-MTAB-8770 | biostudies-arrayexpress

RNA-seq in Raji cells with inducible BZLF1 prior to and after induction of EBV's lytic cycle by doxycycline

Project description:(i) RNA transcripts of Raji iBZLF1 cells (https://www.biorxiv.org/content/10.1101/573659v1) were compared between non-induced cells and cells induced with doxycycline (100 ng/ ml) for 6 h. Human (Hg19) as well as viral (EBV, Raji KF717093.1) transcripts were analyzed. Artificial ERCC Spike-ins (Thermo Scientific) were added to selected samples to control for global changes of RNA levels. (ii) For control purposes, the RNA transcripts in Raji cells equipped with a doxycycline-controlled truncated BZLF1 allele lacking its transactivation domain (Raji iBZLF1 AD-truncated) were prior to and after induction for 6 h. (iii) For another control, the RNA transcripts of parental, unmodified Raji cells were analyzed prior to and after doxycycline induction for 6 h. All experiments were performed as triplicates.

2019-01-01 | E-MTAB-7820 | biostudies-arrayexpress

Double splicing mutant leukemia

Project description:Double splicing mutant leukemia

| PRJNA671435 | ENA

MethylC-Seq of idm2 single mutant and idm1idm2 double mutant

Project description:Using MethylC-Seq to provide single-base resolution of DNA methylation status in idm2 single mutant and idm1idm2 double mutant

2014-07-10 | GSE49421 | GEO

MethylC-Seq of idm2 single mutant and idm1idm2 double mutant

Project description:Using MethylC-Seq to provide single-base resolution of DNA methylation status in idm2 single mutant and idm1idm2 double mutant MethylC-Seq: 2 mutants examined, idm2 single mutant (two biological replicates) and idm1idm2 double mutant

2014-07-10 | E-GEOD-49421 | biostudies-arrayexpress

Measuring inter-protein pairwise interaction energies from a single native mass spectrum by double-mutant cycle analysis.

Project description:The strength and specificity of protein complex formation is crucial for most life processes and is determined by interactions between residues in the binding partners. Double-mutant cycle analysis provides a strategy for studying the energetic coupling between amino acids at the interfaces of such complexes. Here we show that these pairwise interaction energies can be determined from a single high-resolution native mass spectrum by measuring the intensities of the complexes formed by the two wild-type proteins, the complex of each wild-type protein with a mutant protein, and the complex of the two mutant proteins. This native mass spectrometry approach, which obviates the need for error-prone measurements of binding constants, can provide information regarding multiple interactions in a single spectrum much like nuclear Overhauser effects (NOEs) in nuclear magnetic resonance. Importantly, our results show that specific inter-protein contacts in solution are maintained in the gas phase.Double mutant cycle (DMC) analyses can provide the interaction energies between amino acids at the interface of protein complexes. Here, the authors determine pairwise interaction energies using high-resolution native mass spectroscopy, offering a straightforward route for the DMC methodology.

| S-EPMC5550451 | biostudies-other

P. aeruginosa/S. aureus wt, fhuG mutant, fhuD1/D2 double mutant interaction

Project description:Manuscript Title: Pyochelin biotransformation shapes bacterial competition LC-MS/MS analysis of P. aeruginosa interaction with S. aureus wildtype, fhuG mutant and fhuD1/D2 double mutant.

2022-04-10 | MSV000089231 | MassIVE

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data