Project description:The existence of most peptide segments of CYTB-187AA to high confidence in five cell lines using mass spectrometry and the identification of CYTB-187AA interacting proteins.
Project description:We performed IP-MS for identification of SETD1A FLOS domain interacting proteins. Proteins were separated by SDS-PAGE, and digested by in-gel digestion protocol.
Project description:Histone variant H2A.Z is a critical player in setting up the chromatin environment that mediates transcription and other activities on chromatin. However, how H2A.Z is incorporated to specific chromatin regions is not clear. To examine the potential role of sequence-specific transcription factors in targeting H2A.Z, we screened for genome-wide H2A.Z-interacting proteins in vivo using a novel technique called bait Protein-Protein Interaction-sequencing (bPPI-seq). Among the hundreds of H2A.Z-interacting proteins identified by bPPI-seq, we show that a zinc-finger transcription factor, Osr1 interacts with H2A.Z both in vitro and in vivo and co-localizes with H2A.Z on chromatin. Knockdown of Osr1 compromised H2A.Z deposition to hundreds of chromatin sites enriched with Osr1 binding motifs. Furthermore, Osr1 and H2A.Z co-regulate the expression of numerous target genes. These results indicate that Osr1 directly interacts with H2A.Z, mediates its incorporation to a large number of target sites and regulates gene expression. Our data indicate that bPPI-seq can be widely applied to identify unbiasedly interacting proteins under physiologic conditions.
Project description:Examine the potential interacting proteins of QQS by IP-MS analysis using Arabidopsis anthers of the QQS-OE and WT at flower stage 12-15.
Project description:To investigate the role of human EXO5 in DNA replication stress and ICL repair we identifiedEXO5 interacting proteins by IP mass-spectrometry analysis.
Project description:To further examined the potential interacting proteins of QQS in pollen development, we performed IP-MS analysis using Arabidopsis anthers of the wild type, 35S:QQS-Flag and 35S:QQS-GFP lines at flower stage 12-15.
Project description:This study was designed to identify putative NCoR1-interacting proteins that might regulate the mitochondrial biogenesis process. To do that, MEF cells were transiently transfected with plasmid expressing either pcDNA-GFP-FLAG (control) or pCMX-NCoR1-FLAG and subjected to co-IP and identification of interacting proteins by liquid chromatography tandem mass spectrometry (LC-MS/MS) (LTQ Velos Orbitrap).