Proteomics

Dataset Information

0

Proteomics of dried blood collected on Mitra devices


ABSTRACT: Venous blood was collected in Li-heparin or EDTA blood tubes and loaded onto 20 uL Mitra devices or separated to plasma as detailed in metadata. Samples were denatured in 5.5% SDC, reduced and alkylated, and digested with TPCK-trypsin followed by acid precipitation. Filtered precipitated were analyzed by direct injection microflow-LC using a Waters ACQUITY, 1 mm x 150 mm ACQUITY Premier column at 100 uL/min with post-column DMSO and solvent divert. MS/MS used an Exploris 480 with staggered/overlapping DIA acquistion. Demultiplexing was performed using HTRMS converter (Biognosys) and data was analyzed with Spectronaut 18 (Biognosys).

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Matthew W. Foster  

PROVIDER: MSV000093615 | MassIVE | Fri Dec 08 20:36:00 GMT 2023

SECONDARY ACCESSION(S): PXD047666

REPOSITORIES: MassIVE

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2022-03-13 | MSV000089053 | MassIVE
2023-01-22 | ST002459 | MetabolomicsWorkbench
2024-01-19 | MSV000093893 | MassIVE
2023-10-12 | MSV000093088 | MassIVE
2022-10-11 | MSV000090513 | MassIVE
2023-12-10 | MSV000093624 | MassIVE
2014-02-14 | PXD000354 | Pride
2011-03-01 | GSE24444 | GEO
2022-05-31 | GSE192353 | GEO
2022-05-31 | GSE192352 | GEO