Proteomics of dried blood collected on Mitra devices
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ABSTRACT: Venous blood was collected in Li-heparin or EDTA blood tubes and loaded onto 20 uL Mitra devices or separated to plasma as detailed in metadata. Samples were denatured in 5.5% SDC, reduced and alkylated, and digested with TPCK-trypsin followed by acid precipitation. Filtered precipitated were analyzed by direct injection microflow-LC using a Waters ACQUITY, 1 mm x 150 mm ACQUITY Premier column at 100 uL/min with post-column DMSO and solvent divert. MS/MS used an Exploris 480 with staggered/overlapping DIA acquistion. Demultiplexing was performed using HTRMS converter (Biognosys) and data was analyzed with Spectronaut 18 (Biognosys).
INSTRUMENT(S): Orbitrap Exploris 480
ORGANISM(S): Homo Sapiens (ncbitaxon:9606)
SUBMITTER: Matthew W. Foster
PROVIDER: MSV000093615 | MassIVE | Fri Dec 08 20:36:00 GMT 2023
SECONDARY ACCESSION(S): PXD047666
REPOSITORIES: MassIVE
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