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Pla2g12b binding proteins in Zebrafish

ABSTRACT: We injected larvae with the wild-type rescue plasmid described above, and performed co-immunoprecipitation and mass spectrometry (co-IP/MS) against the highly specific 3x-FLAG epitope linked to Pla2g12b. The top five most abundant proteins included the core components of the TRL assembly pathway (ApoB and the two Subunits of Mtp (Mttp and Pdi/P4hb)), as well as various chaperone proteins . These interactions suggest that Pla2g12b participates directly in TRL biogenesis, and may promote efficient channeling of lipids to ApoB by linking it more tightly to the transfer protein Mtp.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Danio Rerio (ncbitaxon:7955)

SUBMITTER: Steve Farber

PROVIDER: MSV000093857 | MassIVE | Fri Jan 12 19:05:00 GMT 2024

SECONDARY ACCESSION(S): PXD048516

REPOSITORIES: MassIVE

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