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Analysis of phot2 phosphorylation profiles and interaction partners involved in chloroplast accumulation and avoidance responses in Arabidopsis thaliana

ABSTRACT: The project aims to use photocycle mutants to understand the importance of phot2 autophosphorylation for signaling leading to chloroplast accumulation and avoidance. The second part is focused on the identification of new interacting proteins for phot2. Characterization of proteins important for signaling leading to chloroplast avoidance. PHOT2-GFP wild type and PHOT2-V392L-GFP muteins were immunoprecipitated from leaves of transgenic Arabidopsis plants, either dark-adapted or irradiated with low or high blue light. Proteins identified by Mass Spectrometry as interacting with wild-type PHOT2-GFP and PHOT2-V392L-GFP were compared to identify proteins putatively involved in chloroplast avoidance. Phosphorylation profiles of PHOT2-GFP wild type and PHOT2-V392L-GFP were analyzed to distinguish between phosphorylation sites characteristic for chloroplast accumulation and avoidance.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702)

SUBMITTER: Justyna Labuz

PROVIDER: MSV000094308 | MassIVE | Wed Mar 13 06:14:00 GMT 2024

REPOSITORIES: MassIVE

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