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Coupland et al., Cryo-EM of synaptic vesicles reveals loading-induced V-ATPase dissociation

ABSTRACT: This dataset consists of 4 raw mass spectrometry files and associated peak lists and results files, acquired on a Bruker timsTOF Pro 2 mass spectrometer operated in Data-Dependent Acquisition mode. Synaptic vesicle and cleared rat brain lysate samples were prepared and separated in SDS-PAGE gel, then processed for in-gel protease digestion by Claire Coupland. Mass spectrometric acquisition and database searches were performed by Network Biology Collaborative Centre at the Lunenfeld-Tanenbaum Research Institute. Mass spec results were analyzed by Ji-Young Youn and John Rubinstein. The files are associated with a manuscript by Coupland et al. that determines the structure of synaptic vesicle V-ATPases and their associated proteins using Cryo-EM structural analysis. Mass spectrometry analysis is used to determine the protein components in purified synaptic vesicles. John Rubinstein is the corresponding author for the manuscript and should be contacted for questions regarding this dataset (john.rubinstein@utoronto.ca).

INSTRUMENT(S): timsTOF Pro 2

ORGANISM(S): Rat Rattus Norvegicus (ncbitaxon:10116)

SUBMITTER: John Rubinstein

PROVIDER: MSV000094439 | MassIVE | Sat Mar 30 05:48:00 GMT 2024

SECONDARY ACCESSION(S): PXD051100

REPOSITORIES: MassIVE

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