Online Monitoring and Stable Isotope Tracing of Cancer Associated Volatiles in Murine Model Captures Oxidative Stress Markers in vivo
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ABSTRACT: In this study, we utilized advanced secondary electrospray ionization (SESI) technique coupled with a high-resolution mass spectrometer (HRMS) to non-invasively monitor lung cancer tumor volatiles in a pre-clinical mouse model in real time. Endogenous tracing of glucose metabolism highlighted the gamma-glutamyl cycle as a downstream pathway implicated in lung cancer, driven by an imbalance in glutathione metabolism due to reactive oxygen species (ROS) accumulation. Notably, our study unveiled unique volatile changes associated with gemcitabine and cisplatin treatment, which significantly abrogated tumor growth in vivo.
INSTRUMENT(S): Q Exactive
ORGANISM(S): Mus Musculus (ncbitaxon:10090)
SUBMITTER: Jiangjiang Zhu
PROVIDER: MSV000094503 | MassIVE | Tue Apr 09 12:33:00 BST 2024
REPOSITORIES: MassIVE
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