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DATASET - Mass Spectrometry - Snake venom proteomics of three subspecies of the North African mountain viper (Vipera monticola, Saint-Girons 1954) from Morocco

ABSTRACT: This DATASET collection includes the mass spectrometry files for proteomics venom investigation of three subspecies of the North African mountain viper (Vipera monticola, Saint-Girons 1954) from Morocco. Species list: 1. Vipera monticola monticola 2. Vipera monticola atlantica 3. Vipera monticola saintgironsi Folders 01-03 - BOTTOM-UP PROTEOMICS: The venom pools were investigated by the bottom-up "snake venomics" (labled as SVX) approach and in short: separated by RP-HPLC, followed by SDS-PAGE separation and the single bands were in-gel processed by DTT, IAC and finally o/n tryptic digested. Samples submitted to HPLC-MS/MS. Early peptidic fractions of the first HPLC run were directly submitted to HPLC-MS/MS analytic w/o further gel procession. Folders 01 to 03 include the MS and MS/MS spectra of the snake species 1-3, respectively. Files are included as RAW and MZML format. Used instrument: LTQ Orbitrap XL mass spectrometer (Thermo, Bremen, Germany) with an Agilent 1260 HPLC system (Agilent Technologies, Waldbronn, Germany) using a reversed-phase Grace Vydac 218MS C18 (2.1 x 150 mm; 5 um particle size) column. Modifications: UNIMOD:4 - \"Iodoacetamide derivative.\" Used protein database: Uniprot_8570_serpentes_reviewed_CandIso_2747_entries_230398.fasta

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Vipera Monticola Atlantica Vipera Monticola Saintgironsi Vipera Monticola Monticola Vipera Monticola (ncbitaxon:1588684)

SUBMITTER: Maik Damm

PROVIDER: MSV000094724 | MassIVE | Thu May 09 06:31:00 BST 2024

REPOSITORIES: MassIVE

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