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Raw MS data - Proximity labeling of Sucrosomes

ABSTRACT: Human iPSCs (WTC11 line) expressing Lamp1-APEX were treated with sucrose overnight to form sucrosomes, and half of the sucrose-treated cells were treated with invertase to shrink sucrosomes to cause lysosome tubulation. Proximity labeling was done of the lysosomal surface as 3 technical replicates per condition. Biotinylated proteins were captured with NeutrAvidin Beads and samples were run by SDS-PAGE until they entered 1/4 the gel length. The short lanes were excised and processed for mass spectrometry by dissolving the gel and trypsin-digesting the samples. Label-free quantitation was performed.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Spencer Freeman

PROVIDER: MSV000094949 | MassIVE | Thu Jun 06 09:25:00 BST 2024

REPOSITORIES: MassIVE

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