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Investigating effects of queen exposure to QueenBoost on queen oocytes and hemolymph as well as worker antennae, hemolymph, and hypopharangeal glands

ABSTRACT: Queens were exposed to low doses pyriproxyfen (QueenBoost, which has been previously found to increase egg hatching rates and attentiveness of worker progeny to the queen) orally within queen monitoring cages. After treatment, queens were sampled to obtain proteomics data for hemolymph and mature oocytes (dissected from the ovaries), and workers were sampled to obtain pooled samples of antennae, hypopharangeal glands, and hemolymph. Data from worker antennae and hypopharangeal gland samples were acquired from a single plate, data from worker and queen hemolymph samples were acquired from a second plate, and oocyte data were acquired at a later date from a third plate. All samples were randomized within plates. The hemolymph, antennae, and hypopharangeal gland data were searched together (report_JF-all-tissues file) and the oocyte data were searched separately (report_JF-oocytes file). Data were all searched library-free using DIA-NN with default parameters except "unrelated runs" and "MBR" were selected, the neural network classifier was set to "double-pass mode", and protein inference was set to "protein names from FASTA".

INSTRUMENT(S): timsTOF Pro 2

ORGANISM(S): Apis Mellifera (ncbitaxon:7460)

SUBMITTER: Leonard Foster

PROVIDER: MSV000095498 | MassIVE | Wed Jul 31 12:05:00 BST 2024

REPOSITORIES: MassIVE

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