Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Direct Analysis in Real Time Mass Spectrometry (DART-MS) for Rapid Screening of Carpaine in Carica papaya Leaf Products

ABSTRACT: DART-MS can rapidly detect carpaine in various forms of papaya leaf products. No sample preparation is needed for DART-MS, enhancing efficiency. Analysis with DART-MS takes less than a minute per sample, ideal for rapid screening. Validated DART-MS method demonstrated robust identification with low LOD.

INSTRUMENT(S): DART-TOF-MS and LC-ESI-QTOF

ORGANISM(S): Carica Papaya

SUBMITTER: Rossarin Tansawat

PROVIDER: MSV000095634 | MassIVE | Sat Aug 17 20:01:00 BST 2024

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

GNPS - Direct Analysis in Real Time Mass Spectrometry (DART-MS) for Rapid Screening of Carpaine in Carica papaya Leaf Products

Project description:DART-MS can rapidly detect carpaine in various forms of papaya leaf products. No sample preparation is needed for DART-MS, enhancing efficiency. Analysis with DART-MS takes less than a minute per sample, ideal for rapid screening. Validated DART-MS method demonstrated robust identification with low LOD.

2024-08-17 | MSV000095634 | GNPS

Improved methods for deamination-based m6A detection

Project description:We present an improved version of DART-seq which utilizes a variant of the YTH domain engineered to achieve enhanced m6A recognition in APO1-YTH (D422N). In addition, we develop in vitro DART-seq and show that it performs similarly to cellular DART-seq and can map m6A in any sample of interest using nanogram amounts of total RNA.

2022-05-11 | GSE196416 | GEO

Rapid characterization of hops (Humulus lupulus) using DART-MS and chemometrics

Project description:Rapid characterization of hops (Humulus lupulus) using DART-MS and chemometrics

2022-11-28 | MSV000090797 | MassIVE

Identification and Evaluation of a Phage Cocktail with High Lytic Activity Against Escherichia coli including Extended-Spectrum Beta-Lactamase (ESBL)- Producing Isolates - A Preclinical Study

Project description:IDEAL-EC

| PRJEB52703 | ENA

E-PROT-106

Project description:Global proteome analysis of paired colorectal cancer sample using LC-MS/MS

2023-06-22 | E-PROT-106 | ExpressionAtlas

Rapid Screening for Deleted Form of ?-thalassemia by Real-Time Quantitative PCR.

Project description:BACKGROUND:Thalassemia is the most common single gene disease in human beings. The prevalence rate of ?-thalassemia in Taiwan is approximately 1-3%. Previously methods to reveal and diagnose severe deleted form of ?- or ?-thalassemia were insufficient and inappropriate for prenatal diagnosis. METHODS:A real-time quantitative PCR method was set up for rapid screening of the deleted form of ?-thalassemia. RESULTS:Our results show that ??Ct between deleted form of ?-thalassemia and normal individuals were 1.0674 ± 0.0713. On the contrary, mutation form ?-thalassemia showed no difference with normal healthy control. The HBB/CCR5 ratio for deleted form of ?-thalassemia patients was 0.48, whether normal individuals and mutation form of ?-thalassemia was 1.0. CONCLUSION:This RQ-PCR technique is an alternative rapid screening assay for deleted form of ?-thalassemia. In addition, it could also identify undefined type. Our technique by using RQ-PCR to quantify gene copies is a reliable and time-saving method that can screen deleted form of ?-thalassemia.

| S-EPMC6817179 | biostudies-literature

Proteomic and Direct Analysis in Real Time Mass Spectrometry Analysis of a Native American Ceremonial Hat

Project description:Direct analysis in real time (DART) surface analysis files and LC-MS proteomics files of a Tlingit ceremonial hat. DART was performed to evaluate what was present on the surface and also to help understand connection between two components of the hat. Proteomics was performed on small samples of material from around the hat to characterize the original adornment. The hat is housed at the Smithsonian Natural Museum of Natural History (E74339, E74441). Proteomics data were searched with PEAKS 8.5 and Prosight Lite v1.4 Build 1.4.6

2019-08-13 | MSV000084203 | MassIVE

Gene expression profiling in zebrafish embryos exposed to 3,4-dichloroaniline (3,4-DCA)

Project description:The zebrafish (Danio rerio) embryo toxicity test (DarT) is considered as an alternative to the acute fish toxicity test. However, DarT rarely reveals information on the mechanisms underlying a toxic effect and is relatively insensitive compared to chronic toxic effects. We hypothesized that, by using gene expression profiles as an additional endpoint, it may be possible to increase the sensitivity and predictive value of DarT. Therefore we exposed zebrafish embryos for 48 h to the reference compound 3,4-dichloroaniline and analyzed gene expression patterns with a 14 k oligonucleotide array. Keywords: differential gene expression upon chemical exposure in zebrafish embryos

2006-11-08 | GSE6228 | GEO

Incorporation of In Vitro Techniques for Botanicals Dietary Supplement Safety Assessment – Towards evaluation of Developmental and Reproductive Toxicity (DART)

Project description:As complex mixtures, botanicals present unique challenges when assessing safe use, particularly when endpoint gaps exist that cannot be fully resolved by existing toxicological literature. Obtaining data for developmental and reproductive toxicity (DART) can be particularly difficult as this is a frequent data gap for botanicals. Here we explore in vitro receptor binding and enzyme activity assays as well gene expression studies as alternative tools to inform on DART relevent modes of action. Botanicals suspected to have DART effects in addition to herbs with a significant history of use were tested at biologically relevant doses to probe activity at a molecular level. Additionally, gene expression changes in a number of different cell types were analysed using the connectivity mapping approach (CMap) to identify modes of action through a functional read across approach. Together, these two data streams increase the confidence in predictions of biological mode of action and inform potential DART risk by way of potency comparisons and/or a margin of internal exposure approach.

2020-05-01 | GSE144235 | GEO

Barley leaf sample preparation

Project description:Barley is an important cereal crop all over the world. Detailed molecular characterization of barley provides the basis for development of improved cultivars, stress and drought-resistant plants. We here present an LC-MS/MS-based proteomics study of barley leaf aimed at optimization of methods to achieve efficient and unbiased trypsin digestion of proteins prior to LC-MS/MS based sequencing and quantification of peptides. We evaluated two spin filter-aided sample preparation protocols using either sodium dodecyl-sulphate (SDS) or sodium deoxycholate (SDC), and three in-solution digestion (ISD) protocols using SDC or trichloroacetic acid/acetone precipitation. The proteomics workflow identified up to 1800 barley proteins based on sequencing of up to 7700 peptides per sample. The two spin filter-based protocols provided a 17-38% higher efficiency than the ISD protocols, including more proteins of low abundance. Among the ISD protocols, a simple one-step reduction and S-alkylation method (OP-ISD) was the most efficient for barley leaf sample preparation; it identified and quantified 1500 proteins and displayed higher peptide-to-protein inference ratio and higher average amino acid sequence coverage of proteins. The two spin filter-aided sample preparation protocols are compatible with TMT labeling for quantitative proteomics studies. They exhibited complementary performance as about 30% of the proteins were identified by either one or the other protocol, but also demonstrated a positive bias for membrane proteins when using SDC as detergent. We provide detailed protocols for efficient barley protein sample preparation for LC-MS/MS-based proteomics studies. Spin filter-based protocols are the most efficient for the preparation of barley leaf samples for MS-based proteomics, however, a simple protocol provides comparable results although with different peptide digestion profile.

2018-09-03 | PXD008938 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data