Proteomics

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Comamonas KF-1 PET degradation

ABSTRACT: Cellular and extracellular fractions of Comamonas testosteroni KF-1 grown on PET +/- acetate, labeled via diDO-IPTL (Waldbauer et al. 2017 Analytical Chemistry) for protein expression quantitation

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Comamonas Testosteroni Kf-1 (ncbitaxon:399795)

SUBMITTER: Jacob Waldbauer  

PROVIDER: MSV000095794 | MassIVE |

SECONDARY ACCESSION(S): PXD055638

REPOSITORIES: MassIVE

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Publications

Mechanisms of Polyethylene Terephthalate Pellet Fragmentation into Nanoplastics and Assimilable Carbons by Wastewater <i>Comamonas</i>.

Wilkes Rebecca A RA   Zhou Nanqing N   Carroll Austin L AL   Aryal Ojaswi O   Teitel Kelly P KP   Wilson Rebecca S RS   Zhang Lichun L   Kapoor Arushi A   Castaneda Edgar E   Guss Adam M AM   Waldbauer Jacob R JR   Aristilde Ludmilla L  

Environmental science & technology 20241003 43

<i>Comamonadaceae</i> bacteria are enriched on poly(ethylene terephthalate) (PET) microplastics in wastewaters and urban rivers, but the PET-degrading mechanisms remain unclear. Here, we investigated these mechanisms with <i>Comamonas testosteroni</i>KF-1, a wastewater isolate, by combining microscopy, spectroscopy, proteomics, protein modeling, and genetic engineering. Compared to minor dents on PET films, scanning electron microscopy revealed significant fragmentation of PET pellets, resulting  ...[more]

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