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Heart surfaceome profiling using biotin capture

ABSTRACT: Cell surface proteins (surfaceome) represent key signalling and interaction molecules for therapeutic targeting, biomarker profiling, and cellular phenotyping. Understanding the surfaceome of complex tissues including the heart is essential to investigate membrane composition and function in physiological and pathological states. Here, we present a protein-centric workflow that enables the labelling, capture and detection of the cardiac cell surfaceome using ultra-high-sensitivity mass spectrometry-based proteome analysis in individual murine hearts. By preserving cardiac cell architecture of the neo-native heart, this workflow combines coronary artery perfusion using cleavable biotin and unbiased capture of the surface-accessible heart proteome.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: David Greening

PROVIDER: MSV000095912 | MassIVE |

REPOSITORIES: MassIVE

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Publications

The Proteomic Landscape of the Coronary Accessible Heart Cell Surfaceome.

Inocencio Iasmin I Rai Alin A Donner Daniel D Greening David W DW

Proteomics 20250110

Cell surface proteins (surfaceome) represent key signalling and interaction molecules for therapeutic targeting, biomarker profiling and cellular phenotyping in physiological and pathological states. Here, we employed coronary artery perfusion with membrane-impermeant biotin to label and capture the surface-accessible proteome in the neo-native (intact) heart. Using quantitative proteomics, we identified 701 heart cell surfaceome accessible by the coronary artery, including receptors, cell surfa ...[more]

PMID: 39790063

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