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Antibacterial Monoclonal Antibodies Do Not Disrupt the Intestinal Microbiome or Its Function

ABSTRACT: Antibiotics revolutionized medicine, however, it is now clear that broad-spectrum antibiotics alter the composition and function of the host’s microbiome. The microbiome plays a key role in human health and its perturbation is increasingly recognized as contributing to many human diseases. Wide spread broad-spectrum antibiotic use has also resulted in the emergence of multi-drug resistant pathogens, spurring development of pathogen-specific strategies such as monoclonal antibodies (mAbs) to combat bacterial infection. Not only are pathogen-specific approaches not expected to induce resistance in non-targeted bacteria, but they are hypothesized to have minimal impact on the gut microbiome. Here, we compare the effects of antibiotics, pathogen-specific mAbs or their controls (saline or c-IgG) on the gut microbiome of 7-week-old, female, C57BL/6 mice. The magnitude of change in taxonomic abundance, bacterial diversity, and bacterial metabolites including short chain fatty acids (SCFA) and bile acids in the fecal pellets from mice treated with pathogen-specific mAbs was no different from animals treated with saline or an IgG control. Conversely, dramatic changes were observed in the relative abundance, as well as alpha- and beta-diversity, of the fecal microbiome, and bacterial metabolites in the feces of all antibiotic-treated mice. Taken together, these results indicate that pathogen-specific mAbs do not alter the fecal microbiome like broad-spectrum antibiotics and may represent a safer, more targeted approach to antibacterial therapy.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase

SUBMITTER: Matthew Glover

PROVIDER: MTBLS1257 | MetaboLights | 2021-01-11

REPOSITORIES: MetaboLights

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			Action	DRS
	MTBLS1257	Other
	FILES	Other
	a_MTBLS1257_LC-MS_negative_reverse-phase_metabolite_profiling-1.txt	Txt
	a_MTBLS1257_LC-MS_negative_reverse-phase_metabolite_profiling-3.txt	Txt
	i_Investigation.txt	Txt

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Antibacterial Monoclonal Antibodies Do Not Disrupt the Intestinal Microbiome or Its Function

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